@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001019, author = "Shen, Yue and Jia, Feiyu and Liu, Chongxi and Li, Jiansong and Guo, Siyu and Zhou, Shuyu and Wang, Xiangjing and Xiang, Wensheng", title = "Nonomuraea zeae sp. nov., isolated from the rhizosphere of corn (Zea mays L.)", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "6", pages = "2259-2264", doi = "https://doi.org/10.1099/ijsem.0.001019", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001019", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Nonomuraea zeae sp. nov.", keywords = "polyphasic taxonomy", keywords = "16S rRNA gene", abstract = "A novel actinobacterium, designated strain NEAU-ND5T, was isolated from the rhizosphere of corn (Zea mays L.) collected in Heilongjiang Province, north-east China, and characterized using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain NEAU-ND5T was a member of the genus Nonomuraea , with highest sequence similarities to Nonomuraea jabiensis A4036T (98.29 %), Nonomuraea rosea GW12687T (98.25 %), Nonomuraea candida HMC10T (98.22 %), Nonomuraea rhizophila YIM 67092T (98.04 %) and Nonomuraea kuesteri NRRL B-24325T (98.04 %). Similarities to other type strains of the genus Nonomuraea were lower than 98 %. Morphological and chemotaxonomic properties of strain NEAU-ND5T were also consistent with the description of the genus Nonomuraea . The cell wall contained meso-diaminopimelic acid and the whole-cell sugars were glucose, ribose and madurose. The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylmonomethylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major menaquinones were MK-9(H4), MK-9(H2) and MK-9(H0). The predominant cellular fatty acids were iso-C16:0 and 10-methyl C17:0. A combination of DNA–DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-ND5T was clearly distinguished from its closely related Nonomuraea species. Consequently, it is concluded that strain NEAU-ND5T represents a novel species of the genus Nonomuraea , for which the name Nonomuraea zeae sp. nov. is proposed. The type strain is NEAU-ND5T (=CGMCC 4.7280T=DSM 100528T).", }