1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 66, Issue 6

gen. nov., sp. nov., isolated from seawater Free

Abstract

A Gram-stain-negative, coccoid- or oval-shaped and non-motile bacterial strain, designated MM-10, belonging to the , was isolated from seawater of the Yellow Sea, Korea, and was subjected to a polyphasic taxonomic study. Strain MM-10 grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2–3 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain MM-10 fell within the clade comprising members of the genera and . Strain MM-10 exhibited a 16S rRNA gene sequence similarity of 97.7 % with respect to the type strain of The DNA G+C content of strain MM-10 was 66.0 mol% and its mean level of DNA–DNA relatedness with LMG 24665 was 12.3±1.4 % . Strain MM-10 contained Q-10 as the predominant ubiquinone and C 7 as the major fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. Differential phenotypic properties, together with phylogenetic distinctiveness, demonstrate that strain MM-10 is distinguishable from recognized species of the genera and . On the basis of the data presented, strain MM-10 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is MM-10 (=KCTC 42721=CECT 8898).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Maliponia aquimaris gen. nov., sp. nov. , Proteobacteria , Rhodobacteraceae and seawater
© 2016 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001021
2016-06-10
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/6/2271.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001021&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A. 1993 Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd edn Cambridge: Cambridge University Press; [CrossRef]
     [Google Scholar]
  2. Baumann P., Baumann L. 1981; The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . In The Prokaryotes pp. 1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
     [Google Scholar]
  3. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868 [View Article][PubMed]
     [Google Scholar]
  4. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda tuestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [View Article][PubMed]
     [Google Scholar]
  5. Chen Z., Zhang J., Lei X., Lai Q., Yang L., Zhang H., Li Y., Zheng W., Tian Y., other authors. 2015; Mameliella phaeodactyli sp. nov., a member of the family Rhodobacteraceae isolated from the marine algae Phaeodactylum tricornutum . Int J Syst Evol Microbiol 65:1617–1621 [View Article][PubMed]
     [Google Scholar]
  6. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49:25–68 [CrossRef]
     [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
     [Google Scholar]
  8. Garrity G. M., Bell J. A., Lilburn T. 2005; Family I. Rhodobacteraceae fam. nov. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 2 pp. 161 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
     [Google Scholar]
  9. Gonzalez J. M., Mayer F., Moran M. A., Hodson R. E., Whitman W. B. 1997; Sagittula stellata gen. nov., sp. nov., a lignin-transforming bacterium from a coastal environment. Int J Syst Bacteriol 47:773–780 [View Article][PubMed]
     [Google Scholar]
  10. Komagata K., Suzuki K. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [CrossRef]
     [Google Scholar]
  11. Labrenz M., Collins M. D., Lawson P. A., Tindall B. J., Braker G., Hirsch P. 1998; Antarctobacter heliothermus gen. nov., sp. nov., a budding bacterium from hypersaline and heliothermal Ekho Lake. Int J Syst Bacteriol 48:1363–1372 [View Article][PubMed]
     [Google Scholar]
  12. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Mocrobiol 19:1–67 [CrossRef]
     [Google Scholar]
  13. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184[PubMed]
     [Google Scholar]
  14. Li G. W., Zhang X. Y., Wang C. S., Zhang Y. J., Xu X. W., Liu C., Li H., Liu A., Xu Z., other authors. 2013; Albimonas pacifica sp. nov., isolated from seawater of the Pacific, and emended description of the genus Albimonas . Int J Syst Evol Microbiol 63:3597–3601 [View Article][PubMed]
     [Google Scholar]
  15. Liu J. J., Zhang X. Q., Chi F. T., Pan J., Sun C., Wu M. 2014; Gemmobacter megaterium sp. nov., isolated from coastal planktonic seaweeds. Int J Syst Evol Microbiol 64:66–71 [View Article][PubMed]
     [Google Scholar]
  16. Lucena T., Ruvira M. A., Garay E., Macián M. C., Arahal D. R., Pujalte M. J. 2012; Actibacterium mucosum gen. nov., sp. nov., a marine alphaproteobacterium from Mediterranean seawater. Int J Syst Evol Microbiol 62:2858–2864 [View Article][PubMed]
     [Google Scholar]
  17. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [CrossRef]
     [Google Scholar]
  18. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
     [Google Scholar]
  19. Park S., Jung Y. T., Yoon J. H. 2014; Wenxinia saemankumensis sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 64:1958–1963 [View Article][PubMed]
     [Google Scholar]
  20. Romanenko L. A., Tanaka N., Svetashev V. I., Mikhailov V. V. 2011; Primorskyibacter sedentarius gen. nov., sp. nov., a novel member of the class Alphaproteobacteria from shallow marine sediments. Int J Syst Evol Microbiol 61:1572–1578 [View Article][PubMed]
     [Google Scholar]
  21. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
     [Google Scholar]
  22. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942[PubMed]
     [Google Scholar]
  23. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
     [Google Scholar]
  24. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
     [Google Scholar]
  25. Yoon J. H., Lee S. T., Park Y. H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48:187–194 [View Article][PubMed]
     [Google Scholar]
  26. Yoon J. H., Kang K. H., Park Y. H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [View Article][PubMed]
     [Google Scholar]
  27. Zheng Q., Chen C., Yan X. J., Wang Y. N., Zeng Y. H., Hao L. K., He W. H., Jiao N. Z. 2010; Mameliella alba gen. nov., sp. nov., a marine bacterium of the Roseobacter clade in the order Rhodobacterales . Int J Syst Evol Microbiol 60:953–957 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001021
Loading
Maliponia aquimaris gen. nov., sp. nov., isolated from seawater
Int J Syst Evol Microbiol 66, 2271 (2016); https://doi.org/10.1099/ijsem.0.001021
/content/journal/ijsem/10.1099/ijsem.0.001021
/content/journal/ijsem/10.1099/ijsem.0.001021
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error