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Volume 66, Issue 7

sp. nov., an actinobacterium isolated from desert soil Free

Abstract

The taxonomic position of a novel actinobacterium, strain SG1, isolated from a Saharan soil sample collected from Béni-Abbès, Béchar (south-west Algeria), was established by using a polyphasic approach. The micro-organism had morphological and chemical features that were consistent with its classification in the genus . The cell-wall peptidoglycan contained -diaminopimelic acid. The whole-cell sugars contained ribose and glucose, but not madurose. The predominant menaquinones were MK-9(H) and MK-9(H). The polar lipid profile contained diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylhydroxymethylethanolamine, phosphatidylhydroxyethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The predominant cellular fatty acids were C 8, iso-C, 10-methyl C, C 9 and C. 16S rRNA gene sequence similarity analysis supported the classification of the isolate in the genus and indicated that it was related most closely to ‘’ DSM 46724 (99.7 % similarity), DSM 43181 (98.7 %), DSM 43847 (98.6 %) and DSM 45763 (98.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SG1 formed a cluster with its closest relative ‘ DSM 46724. However, DNA–DNA relatedness as well as physiological and chemotaxonomical analyses showed that strain SG1 could be differentiated from its closest phylogenetic relatives. Therefore, it is proposed that strain SG1 should be classified as representing a novel species in the genus , for which the name sp. nov. is proposed. The type strain is SG1 (=DSM 46887=CECT 8961).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): actinobacterium , bacterial diversity , Polyphasic taxonomy , Saharan soil , Streptosporangium and Streptosporangium becharense sp. nov.
© 2016 IUMS
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2016-07-01
2024-04-19
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References

  1. Badji B., Zitouni A., Mathieu F., Lebrihi A., Sabaou N. 2006; Antimicrobial compounds produced by Actinomadura sp. AC104 isolated from an Algerian Saharan soil. Can J Microbiol 52:373–382 [View Article][PubMed]
     [Google Scholar]
  2. Badji B., Mostefaoui A., Sabaou N., Lebrihi A., Mathieu F., Seguin E., Tillequin F. 2007; Isolation and partial characterization of antimicrobial compounds from a new strain Nonomuraea sp. NM94 . J Ind Microbiol Biotechnol 34:403–412 [View Article][PubMed]
     [Google Scholar]
  3. Becker B., Lechevalier M. P., Gordon R. E., Lechevalier H. A. 1964; Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole-cell hydrolysates. Appl Microbiol 12:421–423[PubMed]
     [Google Scholar]
  4. Boubetra D., Sabaou N., Zitouni A., Bijani C., Lebrihi A., Mathieu F. 2012; Taxonomy and chemical characterization of new antibiotics produced by Saccharothrix SA198 isolated from a Saharan soil. Microbiol Res 168:223–230 [View Article][PubMed]
     [Google Scholar]
  5. Boubetra D., Bouras N., Mokrane S., Zitouni A., Schumann P., Spröer C., Sabaou N., Klenk H.-P. 2016; Streptosporangium algeriense sp. nov., an actinobacterium isolated from desert soil. Int J Syst Evol Microbiol 66:1034–1038 [View Article]
     [Google Scholar]
  6. Boudjella H., Bouti K., Zitouni A., Mathieu F., Lebrihi A., Sabaou N. 2006; Taxonomy and chemical characterization of antibiotics of Streptosporangium Sg 10 isolated from a Saharan soil. Microbiol Res 161:288–298 [View Article][PubMed]
     [Google Scholar]
  7. Boudjella H., Bouti K., Zitouni A., Mathieu F., Lebrihi A., Sabaou N. 2007; Isolation and partial characterization of pigment-like antibiotics produced by a new strain of Streptosporangium isolated from an Algerian soil. J Appl Microbiol 103:228–236 [View Article][PubMed]
     [Google Scholar]
  8. Boudjella H., Zitouni A., Coppel Y., Mathieu F., Monje M. C., Sabaou N., Lebrihi A. 2010; Antibiotic R2, a new angucyclinone compound from Streptosporangium sp. Sg3 . J Antibiot 63:709–711
     [Google Scholar]
  9. Bouras N., Merrouche R., Lamari L., Mathieu F., Sabaou N., Lebrihi A. 2008; Precursor-directed biosynthesis of new dithiolopyrrolone analogs by Saccharothrix algeriensis NRRL B-24137. Process Biochem 43:1244–1252 [View Article]
     [Google Scholar]
  10. Bouras N., Meklat A., Boubetra D., Saker R., Boudjelal F., Aouiche A., Lamari L., Zitouni A., Schumann P. et al. 2014; Diversity of Actinobacteria in Algerian Saharan soil and description of sixteen new taxa. Revue ElWahat Pour Les Recherches Et Les Etudes 7:67–85
     [Google Scholar]
  11. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
     [Google Scholar]
  12. Chaabane Chaouch F., Bouras N., Mokrane S., Zitouni A., Schumann P., Spröer C., Sabaou N., Klenk H. P. 2016; Streptosporangium saharense sp. nov., an actinobacterium isolated from Saharan soil. Int J Syst Evol Microbiol 66:1371–1376 [View Article][PubMed]
     [Google Scholar]
  13. Cooper R., Das P., Federbush C., Mierzwa R., Patel M., Pramanik B., Truumees I. 1990; Characterization of peptidyl-nucleoside antifungal antibiotics from fermentation broth. J Ind Microbiol 5:1–8 [View Article][PubMed]
     [Google Scholar]
  14. Couch J. N. 1955; A new genus and family of the Actinomycetales, with a revision of the genus Actinoplanes . J Elisha Mitchell Sci Soc 71:148–155
     [Google Scholar]
  15. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
     [Google Scholar]
  16. Fang B., Liu H., Pan T., Liu C., Guan X., He H., Yan K., Li J., Xiang W. et al. 2016; Streptosporangium lutulentum sp. nov., Streptosporangium fenghuangense sp. nov. and Streptosporangium corydalis sp. nov., three novel actinobacterial species isolated from National Forest Park of Fenghuang Mountain. Antonie Van Leeuwenhoek 109:439–448 [View Article][PubMed]
     [Google Scholar]
  17. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
     [Google Scholar]
  18. Felsenstein J. 1985; Confidence Limits on Phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
     [Google Scholar]
  19. Fitch W. M. 1977; On the problem of discovering the most parsimonious tree. Am Nat 111:223–257 [View Article]
     [Google Scholar]
  20. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63 [View Article]
     [Google Scholar]
  21. Hacène H., Boudjellal F., Lefebvre G. 1998; AH7, a non-polyenic antifungal antibiotic produced by a new strain of Streptosporangium roseum . Microbios 96:103–109[PubMed]
     [Google Scholar]
  22. Hayakawa M., Nonomura H. 1987; Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65:501–509 [View Article]
     [Google Scholar]
  23. He H. R., Zhang X. H., Wang H. Y., Liu C. X., Wang S. R., Zhao J. W., Yuan J. H., Wang X. J., Xiang W. S. 2014; Streptosporangium nanhuense sp. nov., a novel actinomycete isolated from soil. Antonie Van Leeuwenhoek 105:1025–1031 [View Article][PubMed]
     [Google Scholar]
  24. Huss V. A., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [View Article][PubMed]
     [Google Scholar]
  25. Inahashi Y., Iwatsuki M., Ishiyama A., Namatame M., Nishihara-Tsukashima A., Matsumoto A., Hirose T., Sunazuka T., Yamada H. et al. 2011a; Spoxazomicins A–C, novel antitrypanosomal alkaloids produced by an endophytic actinomycete, Streptosporangium oxazolinicum K07-0460T. J Antibiot 64:303–307 [View Article]
     [Google Scholar]
  26. Inahashi Y., Matsumoto A., Ōmura S., Takahashi Y. 2011b; Streptosporangium oxazolinicum sp. nov., a novel endophytic actinomycete producing new antitrypanosomal antibiotics, spoxazomicins. J Antibiot 64:297–302 [View Article]
     [Google Scholar]
  27. Intra B., Matsumoto A., Inahashi Y., Ōmura S., Panbangred W., Takahashi Y., Satoshi Ōmura S. 2014; Streptosporangium jomthongense sp. nov., an actinomycete isolated from rhizospheric soil and emendation of the genus Streptosporangium . Int J Syst Evol Microbiol 64:2400–2406 [View Article][PubMed]
     [Google Scholar]
  28. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp. 21–132 Edited by Munro H. N. New York: Academic Press; [CrossRef]
     [Google Scholar]
  29. Kelly K. L., Judd D. B. 1976 Color. Universal Language and Dictionary of Names (National Bureau of Standards Special Publication 440) Washington, DC: US Department of Commerce; [CrossRef]
     [Google Scholar]
  30. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
     [Google Scholar]
  31. Kornsakulkarn J., Saepua S., Supothina S., Chanthaket R., Thongpanchang C. 2014; Sporaridin and sporazepin from actinomycete Streptosporangium sp. BCC 24625. Phytochemistry Letters 10:149–151 [View Article]
     [Google Scholar]
  32. Kroppenstedt R. M. 1982; Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5:2359–2367 [View Article]
     [Google Scholar]
  33. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20 pp. 173–179 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
     [Google Scholar]
  34. Kämpfer P., Glaeser S. P., Grün-Wollny I., Busse H. J. 2013; Streptosporangium sandarakinum sp. nov. Int J Syst Evol Microbiol 63:2484–2489 [View Article][PubMed]
     [Google Scholar]
  35. Lamari L., Zitouni A., Boudjella H., Badji B., Sabaou N., Lebrihi A., Lefebvre G., Seguin E., Tillequin F. 2002a; New dithiolopyrrolone antibiotics from Saccharothrix sp. SA 233. I. Taxonomy, fermentation, isolation and biological activities. J Antibiot 55:696–701 [View Article]
     [Google Scholar]
  36. Lamari L., Zitouni A., Dob T., Sabaou N., Lebrihi A., Germain P., Seguin. E., Tillequin F. 2002b; New dithiolopyrrolone antibiotics from Saccharothrix sp. SA 233. II. Physicochemical properties and structure elucidation. J Antibiot 55:702–707 [View Article]
     [Google Scholar]
  37. Lazzarini A., Cavaletti L., Toppo G., Marinelli F. 2000; Rare genera of actinomycetes as potential producers of new antibiotics. Antonie Van Leeuwenhoek 78:399–405[PubMed] [CrossRef]
     [Google Scholar]
  38. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [View Article]
     [Google Scholar]
  39. Ma Z., Liu H., Liu C., He H., Zhao J., Wang X., Li J., Wang X., Xiang W. 2015; Streptosporangium sonchi sp. nov. and Streptosporangium kronopolitis sp. nov., two novel actinobacteria isolated from a root of common sowthistle (Sonchus oleraceus L.) and a millipede (Kronopolites svenhedind Verhoeff). Antonie Van Leeuwenhoek 107:1491–1499 [View Article][PubMed]
     [Google Scholar]
  40. Merrouche R., Bouras N., Coppel Y., Mathieu F., Monje M. C., Sabaou N., Lebrihi A. 2010; Dithiolopyrrolone antibiotic formation induced by adding valeric acid to the culture broth of Saccharothrix algeriensis . J Nat Prod 73:1164–1166 [View Article][PubMed]
     [Google Scholar]
  41. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–107 [View Article]
     [Google Scholar]
  42. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  43. Nolan M., Sikorski J., Jando M., Lucas S., Lapidus A., Glavina Del Rio T., Chen F., Tice H., Pitluck S. et al. 2010; Complete genome sequence of Streptosporangium roseum type strain (NI 9100T). Stand Genomic Sci 2:29–37 [View Article][PubMed]
     [Google Scholar]
  44. Quintana E., Goodfellow M.. 2012; Genus I. Streptosporangium . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 5 pp. 1811–1825 Edited by Whitman W. B., Goodfellow M., Kämpfer P., Busse H. J., Trujillo M. E., Ludwig W., Suzuki K., Parte A. New York: Springer;
     [Google Scholar]
  45. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [View Article][PubMed]
     [Google Scholar]
  46. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  47. Sasser M. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. MIDI Technical Note 101 Newark. DE: MIDI Inc;
     [Google Scholar]
  48. Sazak A., Sahin N., Camas M., Guven K., Cetin D., Goodfellow M. 2012; Streptosporangium anatoliense sp. nov., isolated from soil in Turkey. Antonie Van Leeuwenhoek 102:269–276 [View Article][PubMed]
     [Google Scholar]
  49. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
     [Google Scholar]
  50. Sletta H., Degnes K. F., Herfindal L., Klinkenberg G., Fjærvik E., Zahlsen K., Brunsvik A., Nygaard G., Aachmann F. L. et al. 2014; Anti-microbial and cytotoxic 1,6-dihydroxyphenazine-5,10-dioxide (iodinin) produced by Streptosporangium sp. DSM 45942 isolated from the fjord sediment. Microbiol Biotechnol 98:603–610 [View Article]
     [Google Scholar]
  51. Stackebrandt E., Kroppenstedt R. M., Jahnke K. D., Kemmerling C., Gurtler H. 1994; Transfer of Streptosporangium viridogriseum (Okuda, et al. 1966), Streptosporangium viridogriseum subsp. kofuense (Nonomura and Ohara 1969), and Streptosporangium albidum (Furumai, et al. 1968) to Kutzneria gen. nov. as Kutzneria viridogrisea comb. nov., Kutzneria kofuensis comb. nov., and Kutzneria albida comb. nov., respectively, and emendation of the genus Streptosporangium . Int J Syst Bacteriol 44:265–269 [CrossRef]
     [Google Scholar]
  52. Takasawa S., Kawamoto I., Takahashi I., Koakura M., Okachi R., Sato S., Yamamoto M., Sato T., Nara T. 1975; Platomycins A and B I Taxonomy of the producing strain and production, isolation and biological properties of platomycins. J Antibiot 28:656–661 [View Article][PubMed]
     [Google Scholar]
  53. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  54. Vaddavalli R., Gaddam B., Linga V. R. 2015; Streptosporangium terrae sp. nov., a novel actinomycete isolated from the rhizosphere of Callistemon citrinus (Curtis), India. J Antibiot1–6
     [Google Scholar]
  55. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. International Committee on Systematic Bacteriology 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
     [Google Scholar]
  56. Williams S. T., Goodfellow M., Alderson G. 1989; Genus Streptomyces Waksman and Henrici 1943, 339AL . In Bergey’s Manual of Systematic Bacteriology vol. 4 pp 2452–2492 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore, MD: Williams & Wilkins;
     [Google Scholar]
  57. Zhang X., Liu C., Zhang Y., Wang H., Wang S., Li C., Wang X., Xiang W. 2014; Streptosporangium shengliense sp. nov., a novel actinomycete isolated from a lake sediment. Antonie Van Leeuwenhoek 105:237–243 [View Article][PubMed]
     [Google Scholar]
  58. Zhou Y., Liu C., Zhang Y., Li C., Xing J., Li L., Zhou S., Wang X., Xiang W. 2014; Streptosporangium subfuscum sp. nov., isolated from the rhizosphere of marigold (Tagetes erecta L.). Antonie Van Leeuwenhoek 106:1231–1238 [View Article][PubMed]
     [Google Scholar]
  59. Zitouni A., Boudjella H., Mathieu F., Sabaou N., Lebrihi A. 2004; Mutactimycin PR, a new anthracycline antibiotic from Saccharothrix sp. SA 103. I. Taxonomy, fermentation, isolation and biological activities. J Antibiot 57:367–372 [View Article][PubMed]
     [Google Scholar]
  60. Zitouni A., Boudjella H., Lamari L., Badji B., Mathieu F., Lebrihi A., Sabaou N. 2005; Nocardiopsis and Saccharothrix genera in Saharan soils in Algeria: isolation, biological activities and partial characterization of antibiotics. Res Microbiol 156:984–993 [View Article][PubMed]
     [Google Scholar]
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Streptosporangium becharense sp. nov., an actinobacterium isolated from desert soil
Int J Syst Evol Microbiol 66, 2484 (2016); https://doi.org/10.1099/ijsem.0.001077
/content/journal/ijsem/10.1099/ijsem.0.001077
/content/journal/ijsem/10.1099/ijsem.0.001077
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