1887

Abstract

A Gram-stain-negative, rod-shaped bacterium, designated Ar-125, was isolated from Antarctic seawater. It produced carotenoid-like pigments and did not produce Bchl . Ar-125 was positive for hydrolysis of DNA, aesculin, gelatin, starch, Tween 40 and Tween 60. The sole respiratory quinone was MK-6. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid, one unidentified glycolipid and two unidentified lipids. The principal fatty acids were branched-chain fatty acids, including iso-Ciso-C G, summed feature 3 (iso-C 2-OH and/or Cω7), iso-C iso-C 3-OH, iso-C 3-OH and iso-C 3-OH, as well as C. The genomic DNA G+C content was 31.8 mol%. On the basis of 16S rRNA gene sequence analysis, Ar-125 is closely related to the species of the genera (with 16S rRNA gene pairwise sequence similarity of 93.7–96.5 %), (94.3–95.8 %), (94.2–95.7 %), (95.5 %), (93.3–95.4 %), (95.3 %) and (94.8–95.3 %), of the family . Phylogenetic analysis indicated that it represented an independent lineage and that the closest relatives were members of the genus . Differential phenotypic properties and chemotaxonomic differences, together with phylogenetic distinctiveness, revealed that Ar-125 could be differentiated from members of closely related genera. Therefore, it is proposed that Ar-125 represents a novel species in a new genus, for which the name gen. nov., sp. nov. (type strain Ar-125 =CGMCC 1.15295=JCM 30876) is proposed.

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2016-07-01
2024-03-19
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References

  1. Baek K., Lee Y. M., Hwang C. Y., Park H., Jung Y. J., Kim M. K., Hong S. G., Kim J. H., Lee H. K. 2015; Psychroserpens jangbogonensis sp. nov., a psychrophilic bacterium isolated from Antarctic marine sediment. Int J Syst Evol Microbiol 65:183–188 [View Article][PubMed]
    [Google Scholar]
  2. Bercovich A., Vazquez S. C., Yankilevich P., Coria S. H., Foti M., Hernández E., Vidal A., Ruberto L., Melo C. et al. 2008; Bizionia argentinensis sp. nov., isolated from surface marine water in Antarctica. Int J Syst Evol Microbiol 58:2363–2367 [View Article][PubMed]
    [Google Scholar]
  3. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (Basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148 [CrossRef]
    [Google Scholar]
  4. Bernardet J.-F. 2010; Order I. Flavobacteriales ord. nov. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 4 pp 105 Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B. New York: Springer;
    [Google Scholar]
  5. Bernardet J. F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  6. Bowman J. P., McCammon S. A., Brown J. L., Nichols P. D., McMeekin T. A. 1997; Psychroserpens burtonensis gen. nov., sp. nov., and Gelidibacter algens gen. nov., sp. nov., psychrophilic bacteria isolated from antarctic lacustrine and sea ice habitats. Int J Syst Bacteriol 47:670–677 [View Article][PubMed]
    [Google Scholar]
  7. Bowman J. P., Nichols D. S. 2005; Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp. nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia, Gelidibacter and Gillisia . Int J Syst Evol Microbiol 55:1471–1486 [View Article][PubMed]
    [Google Scholar]
  8. Dong X.-Z., Cai M.-Y. 2001 Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation);
    [Google Scholar]
  9. Farmer J. J. III, Brenner F. W., Cameron D. N., Birkhead K. M. 2005; Genus I. Vibrio Pacini 1854, 411AL. In Bergey’s Manual of Systematic Bacteriology, the Proteobacteria, Part B, the Gammaproteobacteria , 2nd edn. vol. 2 pp 494–546 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  10. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  11. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  12. Hildebrand D. C., Palleroni N. J., Hendson M., Toth J., Johnson J. L. 1994; Pseudomonas flavescens sp. nov., isolated from walnut blight cankers. Int J Syst Bacteriol 44:410–415 [View Article][PubMed]
    [Google Scholar]
  13. Jooste P. J. 1985; The taxonomy and significance of flavobacterium-cytophaga strains from dairy sources. PhD thesis University of the Orange Free State;
    [Google Scholar]
  14. Jung S. Y., Kang S. J., Lee M. H., Lee S. Y., Oh T. K., Yoon J. H. 2005; Gaetbulibacter saemankumensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 55:1845–1849 [View Article][PubMed]
    [Google Scholar]
  15. Kamekura M., Kates M. 1988; Lipids of halophilic archaebacteria. In Halophilic Bacteria II pp 25–54 Edited by Rodriguez-Valera F. Boca Raton: CRC Press;
    [Google Scholar]
  16. Kim H. S., Hyun D. W., Kim P. S., Lee J. Y., Shin N. R., Yun J. H., Whon T. W., Kim M. S., Jung M. J. et al. 2015; Bizionia fulviae sp. nov., isolated from the gut of an egg cockle, F ulvia mutica . Int J Syst Evol Microbiol 65:3066–3072 [View Article][PubMed]
    [Google Scholar]
  17. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  18. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120[PubMed] [CrossRef]
    [Google Scholar]
  19. Kwon K. K., Lee S. J., Park J. H., Ahn T. Y., Lee H. K. 2006; Psychroserpens mesophilus sp. nov., a mesophilic marine bacterium belonging to the family Flavobacteriaceae isolated from a young biofilm. Int J Syst Evol Microbiol 56:1055–1058 [View Article][PubMed]
    [Google Scholar]
  20. Kwon T., Baek K., Lee K., Kang I., Cho J. C. 2014; Formosa arctica sp. nov., isolated from arctic seawater. Int J Syst Evol Microbiol 64:78–82 [View Article][PubMed]
    [Google Scholar]
  21. Lee D. H., Cho S. J., Kim S. M., Lee S. B. 2013; Psychroserpens damuponensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 63:703–708 [View Article][PubMed]
    [Google Scholar]
  22. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  23. Li H., Zhang X. Y., Liu C., Liu A., Qin Q. L., Su H. N., Shi M., Zhou B. C., Chen X. L. et al. 2015; Bizionia arctica sp. nov., isolated from Arctic fjord seawater, and emended description of the genus Bizionia . Int J Syst Evol Microbiol 65:2925–2930 [View Article][PubMed]
    [Google Scholar]
  24. Macián M. C., Pujalte M. J., Márquez M. C., Ludwig W., Ventosa A., Garay E., Schleifer K. H. 2002; Gelidibacter mesophilus sp. nov., a novel marine bacterium in the family Flavobacteriaceae . Int J Syst Evol Microbiol 52:1325–1329 [View Article][PubMed]
    [Google Scholar]
  25. Marmur J. 1961; The formation of hybrid DNA molecules and their use in studies of DNA homologies. J Mol Biol 3:208–218 [View Article][PubMed]
    [Google Scholar]
  26. McBride M. J. 2014; The Family Flavobacteriaceae . In The Prokaryotes - Other Major Lineages of Bacteria and the Archaea, 4th edn. pp. 643–676 Edited by Rosenberg E., DeLong E. F., Lory S., Stackebrandt E., Thompson F. New York: Springer;
    [Google Scholar]
  27. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 479:297–306 [View Article][PubMed]
    [Google Scholar]
  28. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Frolova G. M., Mikhailov V. V., Bae K. S. 2005; Bizionia paragorgiae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the soft coral Paragorgia arborea . Int J Syst Evol Microbiol 55:375–378 [View Article][PubMed]
    [Google Scholar]
  29. Nedashkovskaya O. I., Kim S. B., Vancanneyt M., Snauwaert C., Lysenko A. M., Rohde M., Frolova G. M., Zhukova N. V., Mikhailov V. V. et al. 2006; Formosa agariphila sp. nov., a budding bacterium of the family Flavobacteriaceae isolated from marine environments, and emended description of the genus Formosa . Int J Syst Evol Microbiol 56:161–167 [View Article][PubMed]
    [Google Scholar]
  30. Nedashkovskaya O. I., Vancanneyt M., Kim S. B. 2010; Bizionia echini sp. nov., isolated from a sea urchin. Int J Syst Evol Microbiol 60:928–931 [View Article][PubMed]
    [Google Scholar]
  31. Park S., Lee J. S., Lee K. C., Yoon J. H. 2013a; Formosa undariae sp. nov., isolated from a reservoir containing the brown algae U ndaria pinnatifida . Int J Syst Evol Microbiol 63:4130–4135 [View Article]
    [Google Scholar]
  32. Park S., Lee J. S., Lee K. C., Yoon J. H. 2013b; Geojedonia litorea gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from coastal seawater. Antonie Van Leeuwenhoek 103:1255–1262 [View Article]
    [Google Scholar]
  33. Park S. C., Choe H. N., Baik K. S., Lee K. H., Seong C. N. 2012; Gaetbulibacter aestuarii sp. nov., isolated from shallow coastal seawater, and emended description of the genus Gaetbulibacter . Int J Syst Evol Microbiol 62:150–154 [View Article][PubMed]
    [Google Scholar]
  34. Park Y. D., Baik K. S., Yi H., Bae K. S., Chun J. 2005; Pseudoalteromonas byunsanensis sp. nov., isolated from tidal flat sediment in Korea. Int J Syst Evol Microbiol 55:2519–2523 [View Article][PubMed]
    [Google Scholar]
  35. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  36. Song E. J., Lee M. H., Seo M. J., Yim K. J., Hyun D. W., Bae J. W., Park S. L., Roh S. W., Nam Y. D. 2014; Bizionia psychrotolerans sp. nov., a psychrophilic bacterium isolated from the intestine of a sea cucumber (Apostichopus japonicus). Antonie Van Leeuwenhoek 106:837–844 [View Article][PubMed]
    [Google Scholar]
  37. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  38. Thompson J. D., Higgins D. G., Gibson T. J. 1994; CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  39. Tindall B. J., Sikorski J., Smibert R. M., Kreig N. R. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology, 3rd edn. pp. 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt. T. M., Snyder L. R. Washington DC, USA: ASM Press;
    [Google Scholar]
  40. Wang B., Sun F., Du Y., Liu X., Li G., Lai Q., Luo J., Shao Z. 2010; Meridianimaribacter flavus gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from marine sediment of the south china sea. Int J Syst Evol Microbiol 60:121–127 [View Article][PubMed]
    [Google Scholar]
  41. Wu Y. H., Xu L., Zhou P., Wang C. S., Oren A., Xu X. W. 2015; Brevirhabdus pacifica gen. nov., sp. nov., isolated from deep-sea sediment in a hydrothermal vent field. Int J Syst Evol Microbiol 65:3645–3651 [View Article][PubMed]
    [Google Scholar]
  42. Xu X. W., Wu Y. H., Zhou Z., Wang C. S., Zhou Y. G., Zhang H. B., Wang Y., Wu M. 2007; Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. Int J Syst Evol Microbiol 57:1619–1624 [View Article][PubMed]
    [Google Scholar]
  43. Yang S. J., Cho J. C. 2008; Gaetbulibacter marinus sp. nov., isolated from coastal seawater, and emended description of the genus Gaetbulibacter . Int J Syst Evol Microbiol 58:315–318 [View Article][PubMed]
    [Google Scholar]
  44. Yoon B. J., Oh D. C. 2011; Formosa spongicola sp. nov., isolated from the marine sponge hymeniacidon flavia. Int J Syst Evol Microbiol 61:330–333 [View Article][PubMed]
    [Google Scholar]
  45. Yoon J. H., Kang C. H., Jung Y. T., Kang S. J. 2013a; Bizionia hallyeonensis sp. nov., isolated from seawater in an oyster farm. Int J Syst Evol Microbiol 63:685–690 [View Article][PubMed]
    [Google Scholar]
  46. Yoon J. H., Lee S. Y., Oh T. K. 2013b; Gaetbulibacter lutimaris sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 63:995–1000 [View Article][PubMed]
    [Google Scholar]
  47. Zhang D. C., Margesin R. 2015; Gelidibacter sediminis sp. nov., isolated from a sediment sample of the yellow sea. Int J Syst Evol Microbiol 65:2304–2309 [View Article][PubMed]
    [Google Scholar]
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