RT Journal Article SR Electronic(1) A1 Park, Sooyeon A1 Yoon, Sun Young A1 Jung, Yong-Taek A1 Won, Sung-Min A1 Yoon, Jung-HoonYR 2016 T1 Gramella sediminilitoris sp. nov., isolated from a tidal flat JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 7 SP 2704 OP 2710 DO https://doi.org/10.1099/ijsem.0.001111 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, aerobic, non-spore-forming, motile by gliding and coccoid, ovoid or rod-shaped bacterial strain, designated GHTF-27T, was isolated from a tidal flat at Goheung, South Korea, and subjected to a taxonomic study using a polyphasic approach. The novel strain grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–5.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain GHTF-27T belongs to the genus Gramella . It exhibited 16S rRNA gene sequence similarity values of 97.6–98.3 % to the type strains of Gramella aestuariivivens , Gramella echinicola , Gramella gaetbulicola , Gramella aquimixticola , Gramella aestuarii and Gramella portivictoriae , respectively, and of 93.8–96.7 % to the type strains of the other species of the genus Gramellawith validly published names. Strain GHTF-27T contained MK-6 as the predominant menaquinone and iso-C15 : 0 and iso-C17 : 0 3-OH as the major fatty acids. The major polar lipids of strain GHTF-27T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain GHTF-27T was 39.7 mol% and DNA–DNA relatedness values with the type strains of the six phylogenetically closely related species of the genus Gramellawere 12–28 %. The differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain GHTF-27T is separated from other species of the genus Gramella . On the basis of the data presented, strain GHTF-27T is considered to represent a novel species of the genus Gramella , for which the name Gramella sediminilitorissp. nov. is proposed. The type strain is GHTF-27T (=KCTC 52208T =NBRC 111992T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001111