1887

Abstract

A novel bacterium, designated KJ031, was isolated from a wetland soil sample taken from Jeju island, Republic of Korea. Cells were Gram-stain-negative, curved rod shaped, oxidase- and catalase- positive, motile and facultatively anaerobic. Growth was observed at pH 6.0–8.0 and at 20–37 °C on R2A agar. Comparative analysis of 16S rRNA gene sequences revealed that strain KJ031 is a member of the genus , sharing highest sequence similarities with KBP-21 (96.2 %) and 5YN8-15 (96.0 %). The major fatty acids were summed feature 3 (C ω7 and/or C ω6), C and summed feature 8 (C ω7 and/or C ω6). The predominant respiratory quinone was Q-8. The major polar lipids of strain KJ031 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid, two unidentified phospholipids and one unidentified polar lipid. The DNA G+C content was 59.2 mol%. On the basis of the evidence presented in this study, strain KJ031 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KJ031 (=KCTC 42852 =CECT 8976).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001112
2016-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/7/2711.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001112&mimeType=html&fmt=ahah

References

  1. Brown A. E. 2007 Benson’s Microbiological Applications: Laboratory Manual in General Microbiology New York: McGraw-Hill;
    [Google Scholar]
  2. Collins M. 1985; Analysis of isoprenoid quinones. Methods Microbiol 18:329–366 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  4. Fitch W. M. 1971; Toward defining the course of evolution: Minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  5. Gonzalez J. M., Saiz-Jimenez C. 2002; A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4:770–773[PubMed] [CrossRef]
    [Google Scholar]
  6. Hucker G. J. 1921; A new modification and application of the gram stain. J Bacteriol 6:395–397[PubMed]
    [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  8. Kwon S. W., Kim B. Y., Kim W. G., Yoo K. H., Yoo S. H., Son J. A., Weon H. Y. 2008; Paludibacterium yongneupense gen. nov., sp. nov., isolated from a wetland, Yongneup, in Korea. Int J Syst Evol Microbiol 58:190–194 [View Article][PubMed]
    [Google Scholar]
  9. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 125–175 Edited by Stackebrandt. E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  10. Lovley D. R. 2006; Dissimilatory Fe(III)- and Mn(IV)-reducing prokaryotes. In The Prokaryotes, 3rd edn. vol. 2 pp 635–658 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer. K. H., Stackebrandt E. New York: Springer; [CrossRef]
    [Google Scholar]
  11. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  12. Pruesse E., Peplies J., Glöckner F. O. 2012; SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 28:1823–1829 [View Article][PubMed]
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  14. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  15. Sheu S. Y., Chen Z. H., Young C. C., Chen W. M. 2014; Paludibacterium paludis sp. nov., isolated from a marsh. Int J Syst Evol Microbiol 64:2497–2502 [View Article][PubMed]
    [Google Scholar]
  16. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 44:846–849 [View Article]
    [Google Scholar]
  17. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  18. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 37:463–464 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001112
Loading
/content/journal/ijsem/10.1099/ijsem.0.001112
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error