1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 66, Issue 8

sp. nov., isolated from lagoon sediments Free

Abstract

Two Gram-stain-negative, rod-shaped phototrophic bacteria (designated strains N1 and C7) were isolated from lagoon sediments. Both strains were positive for catalase and oxidase activity. Casein, starch, urea and Tween 20 were hydrolysed by both strains while chitin, gelatin and Tween 80 were not. In both strains, C, C,Cω6/Cω7 and Cω6/ Cω7 were the predominant fatty acids, with minor amounts of C 3-OH, anteiso-C, C, Cω5, C 10-methyl and Cω5. Strains N1 and C7 contained phosphatidylglycerol and phosphatidylethanolamine as major polar lipids with minor amounts of phosphatidylcholine, unidentified lipids and an unidentified phospholipid. The mean genomic DNA G+C content was 70.6±1 mol% and the two strains were closely related (mean DNA–DNA hybridization >90 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the two strains clustered with species of the genus belonging to the family of the class . Strain N1 has a 16S rRNA gene sequence similarity of 99.2 % with ATCC 11166, 99.1 % with JA737 and <96.6 % with other members of the genus . Strain N1 and C7 shared 100 % 16S rRNA gene sequence similarity. DNA– DNA hybridization values between strain N1 and the type strains of the nearest species were clearly below the 70 % threshold. On the basis of phenotypic and genotypic data, it is proposed that strain N1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is N1 (=KEMB 563-471=JCM 31175), and strain C7 is an additional strain of the species.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): novel species , Rhodobacter sediminis and Rhodobacteraceae.
© 2016 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001130
2016-08-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/8/2965.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001130&mimeType=html&fmt=ahah

References

  1. Cappuccino J. G., Sherman N. 1998 Microbiology – A Laboratory Manual, 5th edn. California: Benjamin/Cummings Science Publishing;
     [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Evol Microbiol 39:224–229
     [Google Scholar]
  3. Girija K. R., Sasikala Ch., Ramana Ch. V., Spröer C., Takaichi S., Thiel V., Imhoff J. F. 2010; Rhodobacter johrii sp. nov., an endospore-producing cryptic species isolated from semi-arid tropical soils. Int J Syst Evol Microbiol 60:2099–2107 [View Article][PubMed]
     [Google Scholar]
  4. Hanada S., Takaichi S., Matsuura K., Nakamura K. 2002; Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. Int J Syst Evol Microbiol 52:187–193 [View Article][PubMed]
     [Google Scholar]
  5. Hiraishi A., Hoshino Y. 1984; Distribution of rhodoquinone in Rhodospirillaceae and its taxonomic implications. J Gen Appl Microbiol 30:435–448 [CrossRef]
     [Google Scholar]
  6. Hiraishi A., Hoshino Y., Kitamura H. 1984; Isoprenoid quinone composition in the classification of Rhodospirillaceae . J Gen Appl Microbiol 30:197–210 [CrossRef]
     [Google Scholar]
  7. Imhoff J. F. 1984; Quinones of phototrophic purple bacteria. FEMS Microbiol Lett 25:85–89 [View Article]
     [Google Scholar]
  8. Kim H. S., Srinivasan S., Lee S. S. 2016; Methyloterrigena soli gen. nov., sp. nov., a methanol-utilizing bacterium isolated from chloroethylene-contaminated soil. Int J Syst Evol Microbiol 66:101–106 [View Article][PubMed]
     [Google Scholar]
  9. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
     [Google Scholar]
  10. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120[PubMed] [CrossRef]
     [Google Scholar]
  11. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
     [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
     [Google Scholar]
  13. Oren A., Duker S., Ritter S. 1996; The polar lipid composition of walsby's square bacterium. FEMS Microbiol Lett 138:135–140 [View Article]
     [Google Scholar]
  14. Raj P. S., Ramaprasad E. V., Vaseef S., Sasikala C., Ramana C. V. 2013; Rhodobacter viridis sp. nov., a phototrophic bacterium isolated from mud of a stream. Int J Syst Evol Microbiol 63:181–186 [View Article][PubMed]
     [Google Scholar]
  15. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101 Newark, DE: MIDI Inc;
     [Google Scholar]
  16. Shivani Y., Subhash Y., Tushar L., Sasikala C., Ramana C. V. 2015; Spirochaeta lutea sp. nov., isolated from marine habitats and emended description of the genus Spirochaeta . Syst Appl Microbiol 38:110–114 [View Article][PubMed]
     [Google Scholar]
  17. Srinivas T. N., Kumar P. A., Sasikala C., Ramana C. V., Imhoff J. F. 2007; Rhodobacter vinayakumarii sp. nov., a marine phototrophic alphaproteobacterium from tidal waters, and emended description of the genus Rhodobacter . Int J Syst Evol Microbiol 57:1984–1987 [View Article][PubMed]
     [Google Scholar]
  18. Srinivas T. N. R., Kumar P. A., Sasikala C., Spröer C., Ramana C. V. 2008; Rhodobacter ovatus sp. nov., a phototrophic alphaproteobacterium isolated from a polluted pond. Int J Syst Evol Microbiol 58:1379–1383 [CrossRef]
     [Google Scholar]
  19. Subhash Y., Tushar L., Sasikala C., Ramana C. V. 2013a; Falsirhodobacter halotolerans gen. nov., sp. nov., isolated from dry soils of a solar saltern. Int J Syst Evol Microbiol 63:2132–2137 [CrossRef]
     [Google Scholar]
  20. Subhash Y., Tushar L., Sasikala C., Ramana C. V. 2013b; Erythrobacter odishensis sp. nov. and Pontibacter odishensis sp. nov. isolated from dry soil of a solar saltern. Int J Syst Evol Microbiol 63:4524–4532 [CrossRef]
     [Google Scholar]
  21. Subhash Y., Sasikala C., Ramana C. V. 2013c; Flavobacterium aquaticum sp. nov., isolated from a water sample of a rice field. Int J Syst Evol Microbiol 63:3463–3469 [CrossRef]
     [Google Scholar]
  22. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Bio Evol 30:2725–2729 [CrossRef]
     [Google Scholar]
  23. Trüper H. G., Pfennig N. 1981; Isolation of members of the families Chromatiaceae and Chlorobiaceae . In The Prokaryotes: A Handbook on Habitats, Isolation, and Identification of Bacteria pp. 279–289 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
     [Google Scholar]
  24. Venkata Ramana V., Sasikala C., Ramana C. 2008; Rhodobacter maris sp. nov., a phototrophic alphaproteobacterium isolated from a marine habitat of India. Int J Syst Evol Microbiol 58:1719–1722 [View Article][PubMed]
     [Google Scholar]
  25. Venkata Ramana V., Anil Kumar P., Srinivas T. N., Sasikala C., Ramana C. 2009; Rhodobacter aestuarii sp. nov., a phototrophic alphaproteobacterium isolated from an estuarine environment. Int J Syst Evol Microbiol 59:1133–1136 [View Article][PubMed]
     [Google Scholar]
  26. Venkata Ramana V., Sasikala C., Ramaprasad E. V. V., Venkata Ramana C. 2010; Description of Ectothiorhodospira salini sp. nov. J Gen Appl Microbiol 56:313–319 [CrossRef]
     [Google Scholar]
  27. Xu M., Xin Y., Yu Y., Zhang J., Zhou Y., Liu H., Tian J., Li Y. 2010; Erythrobacter nanhaisediminis sp. nov., isolated from marine sediment of the South China Sea. Int J Syst Evol Microbiol 60:2215–2220 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001130
Loading
Rhodobacter sediminis sp. nov., isolated from lagoon sediments
Int J Syst Evol Microbiol 66, 2965 (2016); https://doi.org/10.1099/ijsem.0.001130
/content/journal/ijsem/10.1099/ijsem.0.001130
/content/journal/ijsem/10.1099/ijsem.0.001130
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error