1887

Abstract

A Gram-stain-negative, rod-shaped bacterial strain, designated S9-10, was isolated from a sediment sample from the Yellow Sea near China. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain S9-10 was a member of the family and formed a distinct lineage. The genomic DNA G+C content of strain S9-10 was 34.2 mol%. The major respiratory quinone was MK-6. The predominant cellular fatty acids were iso-C (21.1 %), iso-CG (16.3 %) and iso-C 3-OH (12.0 %). The polar lipid profile contained phosphatidylethanolamine, aminophospholipid, aminoglycolipid, two unidentified aminolipids and five unidentified polar lipids. On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analyses, strain S9-10 represents a novel species of a novel genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is S9-10 (=DSM 28133 =LMG 28074).

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2016-09-01
2024-03-19
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References

  1. Alarico S., Rainey F. A., Empadinhas N., Schumann P., Nobre M. F., da Costa M. S. 2002; Rubritepida flocculans gen. nov., sp. nov., a new slightly thermophilic member of the α-1 subclass of the Proteobacteria. Syst Appl Microbiol 25:198–206 [View Article][PubMed]
    [Google Scholar]
  2. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. 1996; Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47:39–52 [View Article]
    [Google Scholar]
  3. Bernardet J.-F., Bowman J. P. 2006; The genus Flavobacterium. In The Prokaryotes, a Handbook on the Biology of Bacteria, 3rd edn.. vol. 7 pp 481–531 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y. 2006; An introduction to the family Flavobacteriaceae. In The Prokaryotes, a Handbook on the Biology of Bacteria, 3rd edn.. vol. 7, pp. 455–480 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  5. Bernardet J. F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  6. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917 [View Article][PubMed]
    [Google Scholar]
  7. Bowman J. P., Nichols D. S. 2005; Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp. nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia, Gelidibacter and Gillisia. Int J Syst Evol Microbiol 55:1471–1486 [View Article][PubMed]
    [Google Scholar]
  8. Bowman J. P. 2006; The marine clade of the family Flavobacteriaceae: the genera Aequorivita, Arenibacter, Cellulophaga, Croceibacter, Formosa, Gelidibacter, Gillisia, Maribacter, Mesonia, Muricauda, Polaribacter, Psychroflexus, Psychroserpens, Robiginitalea, Salegentibacter, Tenacibaculum, Ulvibacter, Vitellibacter and Zobellia. In The Prokaryotes, a Handbook on the Biology of Bacteria, 3rd edn.. vol. 7 pp 677–694 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  9. Hameed A., Shahina M., Lin S. Y., Lai W. A., Hsu Y. H., Liu Y. C., Young C. C. 2014; Aquibacter zeaxanthinifaciens gen. nov., sp. nov., a zeaxanthin-producing bacterium of the family Flavobacteriaceae isolated from surface seawater, and emended descriptions of the genera Aestuariibaculum and Gaetbulibacter. Int J Syst Evol Microbiol 64:138–145 [View Article][PubMed]
    [Google Scholar]
  10. Hameed A., Shahina M., Lin S. Y., Lai W. A., Liu Y. C., Hsu Y. H., Cheng I. C., Young C. C. 2014; Robertkochia marina gen. nov., sp. nov., of the family Flavobacteriaceae, isolated from surface seawater, and emended descriptions of the genera Joostella and Galbibacter. Int J Syst Evol Microbiol 64:533–539 [View Article][PubMed]
    [Google Scholar]
  11. Hameed A., Shahina M., Lin S. Y., Liu Y. C., Lai W. A., Young C. C. 2014; Gramella oceani sp. nov., a zeaxanthin-producing bacterium of the family Flavobacteriaceae isolated from marine sediment. Int J Syst Evol Microbiol 64:2675–2681 [View Article][PubMed]
    [Google Scholar]
  12. Jeong S. H., Park M. S., Jin H. M., Lee K., Park W., Jeon C. O. 2013; Aestuariibaculum suncheonense gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from a tidal flat and emended descriptions of the genera Gaetbulibacter and Tamlana. Int J Syst Evol Microbiol 63:332–338 [View Article][PubMed]
    [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  14. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  15. Kwon Y. M., Choi D., Yang S. H., Kwon K. K., Kim S. J. 2014; Hoppeia youngheungensis gen. nov., sp. nov., a member of the Flavobacteriaceae isolated from tidal flat sediment, and emended descriptions of the genus Sediminibacter and Sediminibacter furfurosus. Int J Syst Evol Microbiol 64:2053–2059 [View Article][PubMed]
    [Google Scholar]
  16. Lee S.-Y., Park S., Oh T.-K., Yoon J.-H. 2010; Description of Olleya aquimaris sp. nov., isolated from seawater, and emended description of the genus Olleya Mancuso Nichols et al. 2005. Int J Syst Evol Microbiol 60:887–891 [View Article][PubMed]
    [Google Scholar]
  17. Li H., Zhang X. Y., Liu C., Liu A., Qin Q. L., Su H. N., Shi M., Zhou B. C., Chen X. L. et al. 2015; Bizionia arctica sp. nov., isolated from Arctic fjord seawater, and emended description of the genus Bizionia. Int J Syst Evol Microbiol 65:2925–2930 [View Article][PubMed]
    [Google Scholar]
  18. Liu K., Li S., Jiao N., Tang K. 2014; Gramella flava sp. nov., a member of the family Flavobacteriaceae isolated from seawater. Int J Syst Evol Microbiol 64:165–168 [View Article][PubMed]
    [Google Scholar]
  19. Liu C., Wen X.-R., Zhang X.-Y., Su H.-N., Chen X.-L., Shi M. 2016; Arcticiflavibacter luteus gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from intertidal sand. Int J Syst Evol Microbiol 65:144–149 [View Article]
    [Google Scholar]
  20. Mancuso Nichols C., Bowman J. P., Guezennec J. 2005; Olleya marilimosa gen. nov., sp. nov., an exopolysaccharide-producing marine bacterium from the family Flavobacteriaceae, isolated from the Southern Ocean. Int J Syst Evol Microbiol 55:1557–1561 [View Article][PubMed]
    [Google Scholar]
  21. Margesin R., Gander S., Zacke G., Gounot A. M., Schinner F. 2003; Hydrocarbon degradation and enzyme activities of cold-adapted bacteria and yeasts. Extremophiles 7:451–458 [View Article][PubMed]
    [Google Scholar]
  22. Margesin R., Zhang D. C. 2013; Humitalea rosea gen. nov., sp. nov., an aerobic bacteriochlorophyll-containing bacterium of the family Acetobacteraceae isolated from soil. Int J Syst Evol Microbiol 63:1411–1416 [View Article][PubMed]
    [Google Scholar]
  23. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
    [Google Scholar]
  24. Nedashkovskaya O. I., Kim S. B., Han S. K., Rhee M. S., Lysenko A. M., Rohde M., Zhukova N. V., Frolova G. M., Mikhailov V. V., Bae K. S. 2004; Algibacter lectus gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from green algae. Int J Syst Evol Microbiol 54:1257–1261 [View Article][PubMed]
    [Google Scholar]
  25. Nedashkovskaya O. I., Kim S. B., Han S. K., Snauwaert C., Vancanneyt M., Swings J., Kim K. O., Lysenko A. M., Rohde M. et al. 2005a; Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae. Int J Syst Evol Microbiol 55:49–55 [View Article][PubMed]
    [Google Scholar]
  26. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Frolova G. M., Mikhailov V. V., Bae K. S. 2005b; Bizionia paragorgiae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the soft coral Paragorgia arborea. Int J Syst Evol Microbiol 55:375–378 [View Article][PubMed]
    [Google Scholar]
  27. Park S., Park J. M., Jung Y. T., Kang C. H., Yoon J. H. 2015; Lacinutrix undariae sp. nov., isolated from a brown algae reservoir. Int J Syst Evol Microbiol 65:2696–2701 [View Article][PubMed]
    [Google Scholar]
  28. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  29. Sambrook J., Frisch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  30. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. MIDI Technical Note 101 Newark, DE: MIDI;
    [Google Scholar]
  31. Song J., Choi A., Im M., Joung Y., Yoshizawa S., Cho J.-C., Kogure K. 2015; Aurantivirga profunda gen. nov., sp. nov., isolated from deep-seawater, a novel member of the family Flavobacteriaceae. Int J Syst Evol Microbiol 65:4850–4856 [View Article][PubMed]
    [Google Scholar]
  32. Stolz A., Busse H. J., Kämpfer P. 2007; Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57:572–576 [View Article][PubMed]
    [Google Scholar]
  33. Süßmuth R., Eberspächer J., Haag R., Springer W. 1987 Biochemisch-Mikrobiologisches Praktikum Stuttgart: Georg Thieme Verlag;
    [Google Scholar]
  34. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary gGenetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  35. Tindall B. J. 1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [View Article]
    [Google Scholar]
  36. Tindall B. J. 1990b; Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Letts 66:199–202 [View Article]
    [Google Scholar]
  37. Tindall B. J., Sikorski J., Smibert R. M., Kreig N. R. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology, 3rd edn. pp 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: ASM Press;
    [Google Scholar]
  38. Yoon J. H., Won S. M., Park D. S., Park S. 2014; Seonamhaeicola aphaedonensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from a tidal flat sediment. Int J Syst Evol Microbiol 64:1876–1881 [View Article][PubMed]
    [Google Scholar]
  39. Zhang D. C., Wang H. X., Liu H. C., Dong X. Z., Zhou P. J. 2006; Flavobacterium glaciei sp. nov., a novel psychrophilic bacterium isolated from the China No.1 glacier. Int J Syst Evol Microbiol 56:2921–2925 [View Article][PubMed]
    [Google Scholar]
  40. Zhang D. C., Redzic M., Schinner F., Margesin R. 2011; Glaciimonas immobilis gen. nov., sp. nov., a member of the family Oxalobacteraceae isolated from alpine glacier cryoconite. Int J Syst Evol Microbiol 61:2186–2190 [View Article][PubMed]
    [Google Scholar]
  41. Zhang D. C., Margesin R. 2015; Gelidibacter sediminis sp. nov., isolated from a sediment sample of the Yellow Sea. Int J Syst Evol Microbiol 65:2304–2309 [View Article][PubMed]
    [Google Scholar]
  42. Zhang R. G., Tan X., Zhao X. M., Deng J., Lv J. 2014; Moheibacter sediminis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from sediment, and emended descriptions of Empedobacter brevis, Wautersiella falsenii and Weeksella virosa. Int J Syst Evol Microbiol 64:1481–1487 [View Article][PubMed]
    [Google Scholar]
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