1887

Abstract

A Gram-stain-negative, facultatively anaerobic bacterium, designated WM3, was isolated from surface seawater collected from the East China Sea. Cells were catalase- and oxidase-positive, short rods and motile by means of a single polar flagellum. Growth occurred at 15–43 °C (optimum 37–40 C), pH 5.5–9.5 (optimum pH 6.5–7.5) and with 0.25–9.0 % (w/v) NaCl (optimum 1.0–1.5 %). Chemotaxonomic analysis showed that the respiratory quinone was ubiquinone-8, the major fatty acids included C (23.6 %), Cω7 (26.2 %) and summed feature 3 (Cω7 and/or iso-C 2-OH, 22.1 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain WM3 was most closely related to the genus , sharing the highest 16S rRNA gene sequence similarity of 95.5 % with both KCTC 12756 and DSM 27697. The genomic DNA G+C content of the strain WM3 was 55.8 mol%. On the basis of phenotypic, chemotaxonomic and genotypic characteristics presented in this study, strain WM3 is suggested to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WM3 (=KCTC 42782=CGMCC 1.15341).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001213
2016-09-01
2024-03-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/9/3437.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001213&mimeType=html&fmt=ahah

References

  1. Alfaro-Espinoza G., Ullrich M. S. 2014; Marinobacterium mangrovicola sp. nov., a marine nitrogen-fixing bacterium isolated from mangrove roots of Rhizophora mangle. Int J Syst Evol Microbiol 64:3988–3993 [View Article][PubMed]
    [Google Scholar]
  2. Chang H. W., Nam Y. D., Kwon H. Y., Park J. R., Lee J. S., Yoon J. H., An K. G., Bae J. W. 2007; Marinobacterium halophilum sp. nov., a marine bacterium isolated from the Yellow Sea. Int J Syst Evol Microbiol 57:77–80 [View Article][PubMed]
    [Google Scholar]
  3. Chimetto L. A., Cleenwerck I., Brocchi M., Willems A., De Vos P., Thompson F. L. 2011; Marinobacterium coralli sp. nov., isolated from mucus of coral (Mussismilia hispida). Int J Syst Evol Microbiol 61:60–64 [View Article][PubMed]
    [Google Scholar]
  4. Farmer J. J. III, Hickman-Brenner F. W. 2006; The genera Vibrio andPhotobacterium. In The Prokaryotes: A Handbook on the Biology of Bacteria, 3rd edn. vol. 6 pp 508–563 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  5. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  6. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  7. González J. M., Mayer F., Moran M. A., Hodson R. E., Whitman W. B. 1997; Microbulbifer hydrolyticus gen. nov., sp. nov., andMarinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int J Syst Bacteriol 47:369–376 [View Article][PubMed]
    [Google Scholar]
  8. Huo Y. Y., Xu X. W., Cao Y., Wang C. S., Zhu X. F., Oren A., Wu M. 2009; Marinobacterium nitratireducens sp. nov. andMarinobacterium sediminicola sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 59:1173–1178 [View Article][PubMed]
    [Google Scholar]
  9. Hwang C. Y., Yoon S. J., Lee I., Baek K., Lee Y. M., Yoo K. C., Yoon H. I., Lee H. K. 2016; Marinobacterium profundum sp. nov., a marine bacterium from deep-sea sediment. Int J Syst Evol Microbiol 66:1561–1566 [View Article]
    [Google Scholar]
  10. Kim H., Choo Y. J., Song J., Lee J. S., Lee K. C., Cho J. C. 2007; Marinobacterium litorale sp. nov. in the orderOceanospirillales. Int J Syst Evol Microbiol 57:1659–1662 [View Article][PubMed]
    [Google Scholar]
  11. Kim Y. G., Jin Y. A., Hwang C. Y., Cho B. C. 2008; Marinobacterium rhizophilum sp. nov., isolated from the rhizosphere of the coastal tidal-flat plant Suaeda japonica. Int J Syst Evol Microbiol 58:164–167 [View Article][PubMed]
    [Google Scholar]
  12. Kim H., Oh H.-M., Yang S.-J., Lee J.-S., Hong J.-S., Cho J.-C. 2009a; Marinobacterium marisflavi sp. nov., isolated from a costal seawater. Current Microbiology 58:511–515 [View Article]
    [Google Scholar]
  13. Kim S.-J., Park S.-J., Yoon D.-N., Park B.-J., Choi B.-R., Lee D.-H., Roh Y., Rhee S.-K. 2009b; Marinobacterium maritimum sp. nov., a marine bacterium isolated from Arctic sediment. Int J Syst Evol Microbiol 59:3030–3034 [View Article]
    [Google Scholar]
  14. Kim J. M., Lee S. H., Jung J. Y., Jeon C. O. 2010; Marinobacterium lutimaris sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 60:1828–1831 [View Article][PubMed]
    [Google Scholar]
  15. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  16. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  17. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E. 1988; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Evol Microbiol 38:358–361 [View Article]
    [Google Scholar]
  18. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184[PubMed]
    [Google Scholar]
  19. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. et al. 2004; ARB: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [View Article][PubMed]
    [Google Scholar]
  20. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 479:297–306 [View Article][PubMed]
    [Google Scholar]
  21. Nokhal T. H., Schlegel H. G. 1983; Taxonomic study ofParacoccus denitrificans. Int J Syst Bacteriol 33:26–37 [View Article]
    [Google Scholar]
  22. Park S. C., Baik K. S., Kim M. S., Kim S. S., Kim S. R., Oh M. J., Kim D., Bang B. H., Seong C. N. 2009; Aequorivita capsosiphonis sp. nov., isolated from the green alga Capsosiphon fulvescens, and emended description of the genus Aequorivita. Int J Syst Evol Microbiol 59:724–728 [View Article][PubMed]
    [Google Scholar]
  23. Park S., Jung Y. T., Kim S., Yoon J. H. 2016; Marinobacterium aestuariivivens sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 66:1718–1723 [View Article][PubMed]
    [Google Scholar]
  24. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  25. Satomi M., Kimura B., Hamada T., Harayama S., Fujii T. 2002; Phylogenetic study of the genus Oceanospirillum based on 16S rRNA and gyrB genes: emended description of the genus Oceanospirillum, description of Pseudospirillum gen. nov.,Oceanobacter gen. nov. and Terasakiella gen. nov. and transfer of Oceanospirillum jannaschii and Pseudomonas stanieri to Marinobacterium as Marinobacterium jannaschii comb. nov. and Marinobacterium stanieri comb. nov. Int J Syst Evol Microbiol 52:739–747 [View Article][PubMed]
    [Google Scholar]
  26. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  27. Xu X. W., Wu Y. H., Zhou Z., Wang C. S., Zhou Y. G., Zhang H. B., Wang Y., Wu M. 2007; Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. andHalomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. Int J Syst Evol Microbiol 57:1619–1624 [View Article][PubMed]
    [Google Scholar]
  28. Zhu X.-F., Jia X.-M., Zhang X.-Q., Wu Y.-H., Chen Z.-Y. 2011 Modern Experimental Technique of Microbiology Hangzhou: Zhejiang University Press (English translation);
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001213
Loading
/content/journal/ijsem/10.1099/ijsem.0.001213
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error