@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001223, author = "Jo, Jung Hun and Choi, Gyu-Min and Lee, Soon-Youl and Im, Wan-Taek", title = "Phenylobacterium aquaticum sp. nov., isolated from the reservoir of a water purifier", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "9", pages = "3519-3523", doi = "https://doi.org/10.1099/ijsem.0.001223", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001223", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Phenylobacterium aquaticum", keywords = "polyphasic taxonomy", keywords = "16S rRNA gene sequence", abstract = "A Gram-reaction-negative, strictly aerobic, non-motile, non-spore-forming, rod-shaped bacterial strain designated W2-3-4T was isolated from the reservoir of a water purifier. This bacterium was characterized to determine its taxonomic position by using a polyphasic approach. Strain W2-3-4T grew well at 25–30 °C on nutrient and R2A agars. On the basis of 16S rRNA gene sequence similarity, strain W2-3-4T was shown to belong to the family Caulobacteraceaeand to be related to Phenylobacterium conjunctum FWC21T (98.0 % sequence similarity) and Phenylobacterium haematophilum CCUG 26751T (97.2 %). Lower sequence similarities were found with the type strains of all other recognized members of the genus Phenylobacterium (95.7–97.1 %). The G+C content of the genomic DNA was 68.7 mol%. The major respiratory quinone was Q-10 and the major fatty acids were summed feature 8 (comprising C18 : 1 ω7c and/or C18 : 1 ω6c), C16 : 0, C18 : 1 ω7c 11-methyl and summed feature 3 (comprising C16 : 1 ω7c and/or C16 : 1 ω6c). The polar lipids were phosphatidylglycerol, an unknown phospholipid, four unknown glycolipids and three unidentified polar lipids. DNA–DNA relatedness values between strain W2-3-4Tand its closest phylogenetically neighbours were below 7 %. Strain W2-3-4T could be differentiated genotypically and phenotypically from recognized species of the genus Phenylobacterium . The isolate therefore represents a novel species, for which the name Phenylobacterium aquaticum sp. nov. is proposed, with the type strain W2-3-4T (=KACC 18306T=LMG 28593T).", }