Pseudoalteromonas gelatinilytica sp. nov., isolated from surface seawater Yan, Jing and Wu, Yue-Hong and Meng, Fan-Xu and Wang, Chun-Sheng and Xiong, Shang-Ling and Zhang, Xi-Ying and Zhang, Yu-Zhong and Xu, Xue-Wei and Zhang, De-Min,, 66, 3538-3545 (2016), doi = https://doi.org/10.1099/ijsem.0.001224, publicationName = Microbiology Society, issn = 1466-5026, abstract= Three Gram-stain-negative, rod-shaped bacteria, designated strains NH153T, F-2-11 and M-1-78, were isolated from surface seawater of the South China Sea and the East China Sea. The three isolates were able to grow at 15–45 °C (optimum 28–37 °C), but no growth occurred at 4 or 50 °C. The pH range for growth was pH 5.5–9.5 (optimum pH 7.5–8.5). The isolates required sea salts for growth and growth occurred in the presence of 0–10 % (w/v) NaCl (optimum 3–5 %); no growth occurred in the presence of 12.0, 15.0 or 20.0 % (w/v) NaCl. They were positive for hydrolysis of gelatin and Tween 80. The sole respiratory quinone was ubiquinone-8 (Q-8). The major cellular fatty acids (>10 %) were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH). The major polar lipid components were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid, one unidentified phospholipid and one unidentified lipid. The genomic DNA G+C content of strain NH153T was 41.4 mol%. Based on 16S rRNA gene sequence analysis, the isolates were closely related to the type strain of Pseudoalteromonas shioyasakiensis (98.0–98.6 % sequence similarity). The 16S rRNA gene sequence similarities between the three isolates were 98.8–99.7 %. Phylogenetic analysis indicated that they formed a distinct lineage and clustered with P. shioyasakiensis and Pseudoalteromonas arabiensis. The level of DNA–DNA relatedness among the three isolates was 78.0–85.5 %. Strain NH153T exhibited average nucleotide identity values of 93.4 and 84.2 % with respect to P. shioyasakiensis JCM 18891T and P. arabiensis JCM 17292T, respectively. The genome-to-genome distance analysis revealed that strain NH153T shared 52.4 % DNA relatedness with P. shioyasakiensis JCM 18891T and 28.1 % with P. arabiensis JCM 17292T. On the basis of the phenotypic, genotypic and chemotaxonomic characterizations, as well as phylogenetic inference obtained in this study, strains NH153T, F-2-11 and M-1-78 represent a novel species within the genus Pseudoalteromonas , for which the name Pseudoalteromonas gelatinilytica sp. nov. is proposed. The type strain is NH153T (=CGMCC 1.15370T=DSM 100951T), and F-2-11 (=CGMCC 1.15364=DSM 100953) and M-1-78 (=CGMCC 1.15365=DSM 100952), are additional strains of the species., language=, type=