1887

Abstract

A mesophilic, facultatively anaerobic, facultatively chemolithoautotrophic bacterium, designated strain TC8, was isolated from a sulfidic shallow-water marine gas vent located at Tor Caldara, in the Tyrrhenian Sea, Italy. Cells were Gram-stain-negative curved rods with one or more polar flagella. Cells were approximately 1–1.5 µm in length and 0.6 µm in width. Strain TC8 grew between 20 and 35 °C (optimum 30 °C), with between 5 and 45 g NaCl l (optimum 15–20 g l) and between pH 4.5 and 8.5 (optimum pH 6.0–7.0). The generation time under optimal conditions was 8 h. Strain TC8 was a facultative chemolithoautotroph also capable of using organic substrates as electron donors and carbon sources. Chemolithoautotrophic growth occurred with sulfur and thiosulfate as the electron donors, CO as the carbon source, and nitrate, oxygen (5 %, v/v) and ferric iron as the electron acceptors. Chemoorganoheterotrophic growth occurred with tryptone, peptone, Casamino acids, pyruvate and glycerol as substrates, while chemolithoherotrophic growth occurred with (+)-glucose, sucrose, yeast extract, acetate, lactate, citrate and -glutamine. The G+C content of the genomic DNA was 59.9 mol%. Phylogenetic analysis of the 16S rRNA gene sequence of strain TC8 showed that this organism formed a lineage within the family , which branched separately from the two closest relatives, MV1 (91.25 % similarity) and MMS-1 (90.13 %). Based on phylogenetic, physiological and chemotaxonomic characteristics, it is proposed that the organism represents a novel species of a new genus within the family , gen. nov., sp. nov. The type strain of is TC8 (=DSM 101688=JCM 31027).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001235
2016-09-01
2024-03-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/9/3579.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001235&mimeType=html&fmt=ahah

References

  1. Bazylinski D. A., Williams T. J., Lefèvre C. T., Trubitsyn D., Fang J., Beveridge T. J., Moskowitz B. M., Ward B., Schübbe S. et al. 2013; Magnetovibrio blakemorei gen. nov., sp. nov., a magnetotactic bacterium (Alphaproteobacteria: Rhodospirillaceae) isolated from a salt marsh. Int J Syst Evol Microbiol 63:1824–1833 [View Article][PubMed]
    [Google Scholar]
  2. Darriba D., Taboada G. L., Doallo R., Posada D. 2012; JModelTest 2: more models, new heuristics and parallel computing. Nat Methods 9:772 [View Article][PubMed]
    [Google Scholar]
  3. Garrity G. M., Bell J. A., Lilburn T. 2005; Class I. Alphaproteobacteria class. nov. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol 2 pp 1–574Edited by Brenner D. J., Krieg N. R., Staley J. T. New York: Springer; [CrossRef]
    [Google Scholar]
  4. Gillis M., Vandamme P., Vos P. D., Swings J., Kersters K. 2001; Polyphasic taxonomy. In Bergey’s Manual® Syst Bacteriol pp 43–48 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer; [CrossRef]
    [Google Scholar]
  5. Gouy M., Guindon S., Gascuel O. 2010; Sea View version 4: A multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol Biol Evol 27:221–224 [View Article][PubMed]
    [Google Scholar]
  6. Guindon S., Gascuel O. 2003; A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704 [View Article][PubMed]
    [Google Scholar]
  7. Imhoff J. F. 1988; Anoxygenic phototrophic bacteria. In Methods Inaquatic Bacteriology pp 207–240 Edited by Austin B. New York: Wyley;
    [Google Scholar]
  8. Inagaki F., Takai K., Nealson K. H., Horikoshi K. 2004; Sulfurovum lithotrophicum gen. nov., sp. nov., a novel sulfur-oxidizing chemolithoautotroph within the epsilon-Proteobacteria isolated from Okinawa Trough hydrothermal sediments. Int J Syst Evol Microbiol 54:1477–1482 [View Article][PubMed]
    [Google Scholar]
  9. Labrenz M., Collins M. D., Lawson P. A., Tindall B. J., Braker G., Hirsch P. 1998; Antarctobacter heliothermus gen. nov., sp. nov., a budding bacterium from hypersaline and heliothermal Ekho Lake. Int J Syst Evol Microbiol 48:1363–1372 [View Article][PubMed]
    [Google Scholar]
  10. Liu Y., Jin J. H., Liu Y. H., Zhou Y. G., Liu Z. P. 2010; Dongia mobilis gen. nov., sp. nov., a new member of the family Rhodospirillaceae isolated from a sequencing batch reactor for treatment of malachite green effluent. Int J Syst Evol Microbiol 60:2780–2785 [View Article][PubMed]
    [Google Scholar]
  11. Mesbah M., Premachandran U., Whitman W. B., Mesbah M., Premachandran U., Whitman W. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  12. Pfennig N., Trüper H. G. 1971; Type and neotype strains of the species of phototrophic bacteria maintained in pure culture. Int J Syst Evol Microbiol 21:19–24
    [Google Scholar]
  13. Rahalkar M., Bahulikar R. A., Deutzmann J. S., Kroth P. G., Schink B. 2012; Elstera litoralis gen. nov., sp. nov., isolated from stone biofilms of lake constance, Germany. Int J Syst Evol Microbiol 62:1750–1754 [View Article][PubMed]
    [Google Scholar]
  14. Sylvan J. B., Toner B. M., Edwards K. J. 2012; Life and death of deep-sea vents: bacterial diversity and ecosystem succession on inactive hydrothermal sulfides. MBio 3:e0027911 [View Article][PubMed]
    [Google Scholar]
  15. Tindall B. J. 1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [View Article]
    [Google Scholar]
  16. Tindall B. J. 1990b; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
    [Google Scholar]
  17. Vetriani C., Speck M. D., Ellor S. V., Lutz R. A., Starovoytov V. 2004; Thermovibrio ammonificans sp. nov., a thermophilic, chemolithotrophic, nitrate-ammonifying bacterium from deep-sea hydrothermal vents. Int J Syst Evol Microbiol 54:175–181 [View Article][PubMed]
    [Google Scholar]
  18. Williams T. J., Lefèvre C. T., Zhao W., Beveridge T. J., Bazylinski D. A. 2012; Magnetospira thiophila gen. nov., sp. nov., a marine magnetotactic bacterium that represents a novel lineage within the Rhodospirillaceae (Alphaproteobacteria). Int J Syst Evol Microbiol 62:2443–2450 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001235
Loading
/content/journal/ijsem/10.1099/ijsem.0.001235
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error