1887

Abstract

Five actinomycete isolates, CPB1-1, CPB2-10, BM1-4, CPB3-1 and CPB1-18, belonging to the genus were isolated from marine sediments collected from Chumphon Province, Thailand. They produced open loops of warty spore chains on aerial mycelia. -Diaminopimelic acid, glucose and ribose were found in their whole-cell hydrolysates. Polar lipids found were diphosphatidylglycerol, phosphatidylethanolamine, lysophosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. Menaquinones were MK-9(H), MK-9(H), MK-10(H) and MK-10(H). Major cellular fatty acids were anteiso-C, anteiso-C and iso-C. The taxonomic position of the strains was described using a polyphasic approach. analysis of the 16S rRNA gene sequence revealed that these five strains exhibited the highest similarities with ‘' GY1 (99.0 %), GIMN4.003 (98.6 %), RC 1831 (98.5 %) and R97 (98.3 %). However, their phenotypic characteristics and 16S rRNA gene sequences as well as DNA–DNA relatedness differentiated these five strains from the other species of the genus . Here, we propose the novel actinomycetes all being representatives of the same novel species, , with type strain CPB1-1 (=JCM 18519=PCU 343=TISTR 2344).

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2016-09-01
2024-03-19
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References

  1. Arai T. 1975 Culture Media for Actinomycetes Tokyo, Japan: The Society for Actinomycetes;
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [View Article][PubMed]
    [Google Scholar]
  3. Endoh R., Suzuki M., Benno Y., Futai K. 2008; Candida kashinagacola sp. nov., C. pseudovanderkliftii sp. nov. and C. vanderkliftii sp. nov., three new yeasts from ambrosia beetle-associated sources. Antonie Van Leeuwenhoek 94:389–402 [CrossRef]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  5. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  7. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  8. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98 NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  9. Hu H., Lin H.-P., Xie Q., Li L., Xie X.-Q., Hong K. 2012; Streptomyces qinglanensis sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 62:596–600 [View Article][PubMed]
    [Google Scholar]
  10. Kämpfer P. 2012; Genus I. Streptomyces Waksman and Henrici 1943, 339AL. emend. Witt and Stackebrandt 1990, 370 emend. Wellington, Stackebrandt, Sanders, Wolstrup and Jorgensen 1992, 159. In Bergey’s Manual of Systematic Bacteriology Part B , 2nd edn. vol. 5 pp 1455–1767 Edited by Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Suzuki K.-I., Ludwig W., Whitman W. B. New York: Springer; [CrossRef]
    [Google Scholar]
  11. Kelly K. L. 1964 Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
    [Google Scholar]
  12. Khan S. T., Tamura T., Takagi M., Shin-Ya K. 2010; Streptomyces tateyamensis sp. nov., Streptomyces marinus sp. nov. and Streptomyces haliclonae sp. nov., isolated from the marine sponge Haliclona sp. Int J Syst Evol Microbiol 60:2775–2779 [View Article][PubMed]
    [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  14. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–148 Edited by Stackebrandt E., Stackebrandt M. Chichester: Wiley;
    [Google Scholar]
  15. Lechevalier M. P., Lechevalier H. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [View Article]
    [Google Scholar]
  16. Lechevalier M. P., De Bievre C., Lechevalier H. 1977; Chemotaxonomy of aerobic actinomycetes: Phospholipid composition. Biochem Syst Ecol 5:249–260 [View Article]
    [Google Scholar]
  17. Lee L.-H., Zainal N., Azman A.-S., Eng S.-K., Mutalib N.-S., Yin W.-F., Chan K.-G. 2014; Streptomyces pluripotens sp. nov., a bacteriocin-producing streptomycete that inhibits meticillin-resistant Staphylococcus aureus . Int J Syst Evol Microbiol 64:3297–3306 [View Article][PubMed]
    [Google Scholar]
  18. Liu C., Wang X., Yan Y., Wang J., Zhang B., Zhang J., Xiang W. 2013; Streptomyces heilongjiangensis sp. nov., a novel actinomycete that produces borrelidin isolated from the root surface of soybean [Glycine max (L.) Merr]. Int J Syst Evol Microbiol 63:1030–1036 [View Article][PubMed]
    [Google Scholar]
  19. Mao J., Tang Q., Zhang Z., Wang W., Wei D., Huang Y., Liu Z., Shi Y., Goodfellow M. 2007; Streptomyces radiopugnans sp. nov., a radiation-resistant actinomycete isolated from radiation-polluted soil in China. Int J Syst Evol Microbiol 57:2578–2582 [View Article][PubMed]
    [Google Scholar]
  20. Mingma R., Duangmal K., Thamchaipenet A., Trakulnaleamsai S., Matsumoto A., Takahashi Y. 2015; Streptomyces oryzae sp. nov., an endophytic actinomycete isolated from stems of rice plant. J Antibiot 68:368–372 [View Article][PubMed]
    [Google Scholar]
  21. Minnikin D. E., Patek V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [View Article]
    [Google Scholar]
  22. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  23. Phongsopitanun W., Thawai C., Suwanborirux K., Kudo T., Ohkuma M., Tanasupawat S. 2014; Streptomyces chumphonensis sp. nov., isolated from marine sediments. Int J Syst Evol Microbiol 64:2605–2610 [View Article][PubMed]
    [Google Scholar]
  24. Raeder U., Broda P. 1985; Rapid preparation of DNA from filamentous fungi. Lett Appl Microbiol 1:17–20 [View Article]
    [Google Scholar]
  25. Ray L., Mishra S. R., Panda A. N., Rastogi G., Pattanaik A. K., Adhya T. K., Suar M., Raina V. 2014; Streptomyces barkulensis sp. nov., isolated from an estuarine lake. Int J Syst Evol Microbiol 64:1365–1372 [View Article][PubMed]
    [Google Scholar]
  26. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  27. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  28. Ser H.-L., Palanisamy U. D., Yin W.-F., Abd Malek S. N., Chan K.-G., Goh B.-H., Lee L.-H. 2015; Presence of antioxidative agent, Pyrrolo[1, 2-a]pyrazine-1, 4-dione, hexahydro- in newly isolated Streptomyces mangrovisoli sp. nov. Front Microbiol 6:854 [View Article][PubMed]
    [Google Scholar]
  29. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
    [Google Scholar]
  30. Sorokin D. Y. 2005; Is there a limit for high-pH life?. Int J Syst Evol Microbiol 55:1405–1406 [View Article][PubMed]
    [Google Scholar]
  31. Sripreechasak P., Matsumoto A., Suwanborirux K., Inahashi Y., Shiomi K., Tanasupawat S., Takahashi Y. 2013; Streptomyces siamensis sp. nov., and Streptomyces similanensis sp. nov., isolated from Thai soils. J Antibiot 66:633–640 [View Article][PubMed]
    [Google Scholar]
  32. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155
    [Google Scholar]
  33. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231[PubMed]
    [Google Scholar]
  34. Suriyachadkun C., Chunhametha S., Thawai C., Tamura T., Potacharoen W., Kirtikara K., Sanglier J. 2009; Planotetraspora thailandica sp. nov., isolated from soil in Thailand. Int J Syst Evol Microbiol 59:992–997 [View Article][PubMed]
    [Google Scholar]
  35. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  36. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  37. Tomiyasu I. 1982; Mycolic acid composition and thermally adaptative changes in Nocardia asteroides. J Bacteriol 151:828–837[PubMed]
    [Google Scholar]
  38. Trujillo M. E., Alonso-Vega P., Rodríguez R., Carro L., Cerda E., Alonso P., Martínez-Molina E. 2010; The genus Micromonospora is widespread in legume root nodules: the example of Lupinus angustifolius . ISME J 4:1265–1281 [View Article][PubMed]
    [Google Scholar]
  39. Uchida K., Aida K. 1984; An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 37:463–464
    [Google Scholar]
  40. Versalovic J., Schneider M., De Brujin F. J., Lupski J. 1994; Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Meth Mol Cell Biol 5:25–40
    [Google Scholar]
  41. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematic. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  42. Williams S. T., Cross T. 1971; Chapter XI Actinomycetes. Methods Microbiol 4:295–334 [CrossRef]
    [Google Scholar]
  43. Xu L., Li Q., Jiang C. 1996; Diversity of soil actinomycetes in Yunnan, China. Appl Envion Microbiol 62:244–248
    [Google Scholar]
  44. Xu L. H., Tiang Y. Q., Zhang Y. F., Zhao L. X., Jiang C. L. 1998; Streptomyces thermogriseus, a new species of the genus Streptomyces from soil, lake and hot-spring. Int J Syst Bacteriol 48:1089–1093 [View Article][PubMed]
    [Google Scholar]
  45. Yousif G., Busarakam K., Kim B.-Y., Goodfellow M. 2015; Streptomyces mangrovi sp. nov., isolated from mangrove forest sediment. Antonie Van Leeuwenhoek 108:783–791 [View Article][PubMed]
    [Google Scholar]
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