RT Journal Article SR Electronic(1) A1 Huang, Huiqin A1 Feng, Fengzhao A1 Liu, Min A1 Zhang, Fute A1 Sun, Qianguang A1 Qin, Songyu A1 Bao, ShixiangYR 2016 T1 Paenibacillus segetis sp. nov., isolated from soil of a tropical rainforest JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 9 SP 3703 OP 3707 DO https://doi.org/10.1099/ijsem.0.001258 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-positive, facultatively anaerobic, endospore-forming, irregular rod-shaped bacterium, designated DB13260T, was isolated from tropical rainforest soil in Jianfengling Nature Reserve in Hainan, China. The isolate was found to grow with 0–4 % (w/v) NaCl, at 5–40 °C and pH 6.0–10.5, with an optimum of 0 % NaCl, 30–37 °C and pH 8.5–9.0, respectively. The predominant isoprenoid quinone was menaquinone 7 (MK-7), and the major fatty acids were anteiso-C15 : 0, iso-C16 : 0 and C16 : 0. The G+C content of the genomic DNA was 53.7 mol%. Analysis of the 16S rRNA gene sequence of strain DB13260T showed an affiliation of the strain with the genus P aenibacillus , sharing 98.3 % and 97.8 % 16S rRNA gene sequence similarities with the closest relatives P aenibacillus anaericanus MH21T and Paenibacillus selenii W126T, respectively. The DNA–DNA hybridization values between strain DB13260T and the two type strains were 60.4 % and 42.6 %, respectively. The combined phenotypic and DNA–DNA hybridization data supported the conclusion that strain DB13260T represents a novel species of the genus Paenibacillus , for which the name Paenibacillus segetis sp. nov. is proposed. The type strain is DB13260T (=CGMCC 1.12769T=DSM 28014T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001258