1887

Abstract

A novel Gram-staining-negative, aerobic and rod-shaped strain designated 541 was isolated from surface-sterilized root tissue of maize, collected from the Fangshan District of Beijing, People’s Republic of China, and was subjected to a taxonomic study using a polyphasic approach. According to a phylogenetic tree based on 16S rRNA gene sequences, strain 541 represented a member of the genus and clustered with DSM 19645, with which it shared the highest 16S rRNA gene sequence similarity (98.8 %). The predominant respiratory quinone was ubiquinone-10 (Q-10), the major polyamine was sym-homospermidine and the major cellular fatty acids were Cω7 (50.9 %), C (22.0 %) and C 2-OH (11.4 %). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sphingoglycolipid. The DNA G+C content was 64.7 mol%. DNA–DNA relatedness between strain 541 and its closest phylogenetic relative DSM 19645 was 50.8 %. The results of physiological and biochemical tests and the differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain 541 from closely related species of the genus Strain 541 represents a novel species within the genus , for which the name sp. nov. is proposed, with the type strain 541 (=CGMCC 1.15008=DSM 100587).

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2016-09-01
2024-03-19
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