@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001271, author = "Subhash, Y. and Bang, John J. and You, Taek H. and Lee, Sang-Seob", title = "Roseomonas rubra sp. nov., isolated from lagoon sediments", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "10", pages = "3821-3827", doi = "https://doi.org/10.1099/ijsem.0.001271", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001271", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Roseomonas rubra", keywords = "Acetobacteraceae", keywords = "novel species", abstract = "A novel Gram-stain-negative, coccus to oval-shaped, non-motile bacterium, designated strain S5T, was isolated from lagoon sediments collected from North Carolina, USA. Strain S5T was able to grow at 12–45 °C (optima, 30–37 °C) and at pH 6.3–9.0 (optima, 6.5–7.5). No added NaCl was required for growth of strain S5T. Strain S5T was positive for catalase and oxidase activity. C18 : 1 ω6c/C18 : 1 ω7c, C16 : 1 ω6c/C16 : 1 ω7c and C16 : 0 were predominant fatty acids with minor amounts of C8 : 0 3-OH, C14 : 0, C16 : 0 3-OH, C18 : 1 2-OH, C14 : 1 ω5c, C16 : 1 ω5c and C19 : 0 cyclo ω8c. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and an unidentified glycolipid were major polar lipids. Minor amounts of an unidentified amino lipid and three unidentified lipids were also detected. The G+C content of the genomic DNA was 73.5 mol%. 16S rRNA gene sequence comparisons indicated that strain S5T represents a member of the genus Roseomonas within the family Acetobacteraceae of the class Alphaproteobacteria . Strain S5T had a sequence similarity of 97.80 % with Roseomonas rhizosphaerae YW11T, 97.69 % with Roseomonas aestuarii JC17T and <97 % with other members of the genus Roseomonas . However, strain S5T showed only 45.2±2 and 17±2 % relatedness (based on DNA–DNA hybridization) with R . rhizosphaerae KACC 17225T (=YW11T) and R . aestuarii KCTC 22692T (=JC17T), respectively. Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strain S5T as a representative of a novel species in the genus Roseomonas , for which the name Roseomonas rubra sp. nov. is proposed. The type strain is S5T (=KEMB 563-468T=JCM 31177T).", }