@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001279, author = "Tohno, Masanori and Sakamoto, Mitsuo and Ohkuma, Moriya and Tajima, Kiyoshi", title = "Paenibacillus silagei sp. nov. isolated from corn silage", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "10", pages = "3873-3877", doi = "https://doi.org/10.1099/ijsem.0.001279", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001279", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "New Taxa", keywords = "Paenibacillus", keywords = "corn silage", abstract = "A Gram-stain-negative, rod-shaped, endospore-forming bacterium, strain LOOC204T, was isolated from corn silage that had been prepared and stored in Hokkaido, Japan. The isolate grew well at 4–37 °C and at pH 6.0–8.5. The major cellular fatty acids were anteiso-C15 : 0, C16 : 0, iso-C14 : 0 and iso-C16 : 0. The major menaquinone was MK-7. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminophospholipids, one unidentified phospholipid, two unidentified aminolipids and three unidentified lipids were detected as the cellular polar lipids. The genomic DNA G+C content of LOOC204T was 52.2±0.7 mol%. A comparative 16S rRNA gene sequence analysis showed that LOOC204T represented a member of the genus Paenibacillus , and high levels of sequence similarity of LOOC204T with Paenibacillus borealis KK19T (97.5 %) and Paenibacillus jilunlii Be17T (97.4 %) were found. Based on sequence analysis of the RNA polymerase β subunit (rpoB) gene, LOOC204T was clearly separated from its phylogenetic neighbors in the genus Paenibacillus . DNA–DNA hybridisation experiments showed that LOOC204T displayed less than 40 % reassociation values toward its nearest phylogenetic neighbours of the genus Paenibacillus , indicating the unique taxonomic status of LOOC204T. Based on these data, we conclude that strain LOOC204T represents a novel species of the genus Paenibacillus , named Paenibacillus silagei sp. nov. The type strain is LOOC204T (=JCM 30974T=DSM 101953T).", }