RT Journal Article SR Electronic(1) A1 Tohno, Masanori A1 Sakamoto, Mitsuo A1 Ohkuma, Moriya A1 Tajima, KiyoshiYR 2016 T1 Paenibacillus silagei sp. nov. isolated from corn silage JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 10 SP 3873 OP 3877 DO https://doi.org/10.1099/ijsem.0.001279 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, rod-shaped, endospore-forming bacterium, strain LOOC204T, was isolated from corn silage that had been prepared and stored in Hokkaido, Japan. The isolate grew well at 4–37 °C and at pH 6.0–8.5. The major cellular fatty acids were anteiso-C15 : 0, C16 : 0, iso-C14 : 0 and iso-C16 : 0. The major menaquinone was MK-7. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminophospholipids, one unidentified phospholipid, two unidentified aminolipids and three unidentified lipids were detected as the cellular polar lipids. The genomic DNA G+C content of LOOC204T was 52.2±0.7 mol%. A comparative 16S rRNA gene sequence analysis showed that LOOC204T represented a member of the genus Paenibacillus , and high levels of sequence similarity of LOOC204T with Paenibacillus borealis KK19T (97.5 %) and Paenibacillus jilunlii Be17T (97.4 %) were found. Based on sequence analysis of the RNA polymerase β subunit (rpoB) gene, LOOC204T was clearly separated from its phylogenetic neighbors in the genus Paenibacillus . DNA–DNA hybridisation experiments showed that LOOC204T displayed less than 40 % reassociation values toward its nearest phylogenetic neighbours of the genus Paenibacillus , indicating the unique taxonomic status of LOOC204T. Based on these data, we conclude that strain LOOC204T represents a novel species of the genus Paenibacillus , named Paenibacillus silagei sp. nov. The type strain is LOOC204T (=JCM 30974T=DSM 101953T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001279