1887

Abstract

A novel sulfate-reducing bacterium, strain J2, was isolated from a serpentinized peridotite sample from the Indian Ocean. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J2 clustered with the genus within the family , but it showed low similarity (87.95 %) to the type species DSM 642. It was most closely related to MSL79 (96.96 %), followed by Aspo-2 (96.11 %), C1TLV30 (96.04 %) and DSM 11384 (95.17 %). Other available sequences shared less than 93.33 % 16S rRNA gene sequence similarity. Cells were Gram-staining-negative, anaerobic, motile vibrios (2–6×0.4–0.6 µm). Growth was observed at salinities ranging from 0.2 to 6 % (optimum 2.5 %), from pH 5 to 8 (optimum pH 6.5–7) and at temperatures between 9 and 40 °C (optimum 30–35 °C). J2 was piezophilic, growing optimally at 10 MPa (range 0–30 MPa). J2 used lactate, malate, pyruvate, formate and hydrogen as energy sources. Sulfate, thiosulfate, sulfite, fumarate and nitrate were used as terminal electron acceptors. Lactate and pyruvate were fermented. The main fatty acids were iso-C, anteiso-C, summed feature 9 (iso-Cω9 and/or C 10-methyl) and iso-C. The DNA G+C content of strain J2 was 63.5 mol%. The combined genotypic and phenotypic data show that strain J2 represents a novel species of a novel genus in the family , for which the name gen. nov., sp. nov. is proposed, with the type strain J2 (=MCCC 1A01867 = DSM 101483). We also propose the reclassification of as comb. nov., as comb. nov., as comb. nov. and as comb. nov.

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2016-10-01
2024-03-19
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