1887

Abstract

An orange-coloured, aerobic, motile, short-rod-shaped bacterial strain, designated EGI 6500337, was isolated from the surface-sterilized root of a halophyte, (C. A. Mey.) Schischk, collected from Urumqi, Xinjiang province, north-west China. Growth occurred at 5–35 °C (optimum 30 °C), at pH 6.0–9.0 (optimum pH 7.0) and in the presence of 0–6 % (w/v) NaCl (optimum 0–1 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain EGI 6500337 formed a distinct lineage in the cluster that comprised the genera and in the family . The 16S rRNA gene sequence of strain EGI 6500337 shared highest similarity with those of DSM 14790 (97.15 %) and DSM 21871 (97.15 %). Strain EGI 6500337 contained Q-10 as the dominant isoprenoid quinone. The major cellular fatty acids were C 7 and C 8 cyclo. The polar lipid profile of strain EGI 6500337 contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine as major components, similarly to members of the genus . The DNA G+C content of strain EGI 6500337 was 66.8 mol%. The level of DNA–DNA relatedness between strain EGI 6500337 and DSM 14790 was 24.7±2.9 %. On the basis of the phylogenetic analysis, chemotaxonomic data and phenotypic characteristics, strain EGI 6500337 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EGI 6500337 (=KCTC 52296=CPCC 100904).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001320
2016-10-01
2024-03-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/10/4112.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001320&mimeType=html&fmt=ahah

References

  1. Anderson C. R., Dick G. J., Chu M. L., Cho J. C., Davis R. E., Bräuer S. L., Tebo B. M. 2009; Aurantimonas manganoxydans, sp. nov. and Aurantimonas litoralis, sp. nov.: Mn(II) oxidizing representatives of a globally distributed clade of alpha-Proteobacteria from the order Rhizobiales . Geomicrobiol J 26:189–198 [View Article][PubMed]
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria . J Gen Microbiol 100:221–230 [View Article][PubMed]
    [Google Scholar]
  3. Denner E. B., Smith G. W., Busse H. J., Schumann P., Narzt T., Polson S. W., Lubitz W., Richardson L. L. 2003; Aurantimonas coralicida gen. nov., sp. nov., the causative agent of white plague type II on Caribbean scleractinian corals. Int J Syst Evol Microbiol 53:1115–1122 [View Article][PubMed]
    [Google Scholar]
  4. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  6. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  7. Jurado V., Gonzalez J. M., Laiz L., Saiz-Jimenez C. 2006; Aurantimonas altamirensis sp. nov., a member of the order Rhizobiales isolated from Altamira cave. Int J Syst Evol Microbiol 56:2583–2585 [View Article][PubMed]
    [Google Scholar]
  8. Kelly K. L. 1964; Color-name charts illustrated with centroid colors. Inter-Society Color Council-National Bureau of Standards Chicago (Published in US);
    [Google Scholar]
  9. Kim M. S., Hoa K. T., Baik K. S., Park S. C., Seong C. N. 2008; Aurantimonas frigidaquae sp. nov., isolated from a water-cooling system. Int J Syst Evol Microbiol 58:1142–1146 [View Article][PubMed]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  11. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [CrossRef]
    [Google Scholar]
  12. Komagata K., Suzuki K. I 1987; Lipid and cell-wall analysis in bacterial systematics. In Methods in Microbiology Edited by Colwell R. R., Grigorova R. Orlando, Florida: Academic Press;
    [Google Scholar]
  13. Kroppenstedt R. M. 1982; Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded Ion exchanger as stationary phases. J Liq Chromatogr 5:2359–2367 [View Article]
    [Google Scholar]
  14. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematic pp 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  15. Lin S. Y., Hameed A., Liu Y., Hsu Y. H., Lai W. A., Shen F. T., Young L. S., Tsai C. F., Young C. C. 2013; Aureimonas ferruginea sp. nov. and Aureimonas rubiginis sp. nov., two siderophore-producing bacteria isolated from rusty iron plates. Int J Syst Evol Microbiol 63:2430–2435 [View Article][PubMed]
    [Google Scholar]
  16. Madhaiyan M., Hu C. J., Jegan Roy J., Kim S. J., Weon H. Y., Kwon S. W., Ji L. 2013; Aureimonas jatrophae sp. nov. and Aureimonas phyllosphaerae sp. nov., leaf-associated bacteria isolated from Jatropha curcas L. Int J Syst Evol Microbiol 63:1702–1708 [View Article][PubMed]
    [Google Scholar]
  17. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of Deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  19. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  20. Qin S., Wang H. B., Chen H. H., Zhang Y. Q., Jiang C. L., Xu L. H., Li W. J. 2008; Glycomyces endophyticus sp. nov., an endophytic actinomycete isolated from the root of Carex baccans Nees. Int J Syst Evol Microbiol 58:2525–2528 [View Article][PubMed]
    [Google Scholar]
  21. Rathsack K., Reitner J., Stackebrandt E., Tindall B. J. 2011; Reclassification of Aurantimonas altamirensis (Jurado, et al., 2006), Aurantimonas ureilytica (Weon, et al., 2007) and Aurantimonas frigidaquae (Kim, et al., 2008) as members of a new genus, Aureimonas gen. nov., as Aureimonas altamirensis gen. nov., comb nov., Aureimonas ureilytica comb. nov. and Aureimonas frigidaquae comb. nov., and emended descriptions of the genera Aurantimonas and Fulvimarina . Int J Syst Evol Microbiol 61:2722–2728 [CrossRef]
    [Google Scholar]
  22. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  23. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101 Microbial ID, Inc Newark:
    [Google Scholar]
  24. Shirling E. B., Gottlieb D. 1966; Methods for characterization of streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
    [Google Scholar]
  25. Skerman V. B. D. 1967 A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  26. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I, Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  29. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L. 2005; Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae' isolated from China. Int J Syst Evol Microbiol 55:1149–1153 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001320
Loading
/content/journal/ijsem/10.1099/ijsem.0.001320
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error