1887

Abstract

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated, non-gliding and rod-shaped bacterial strain, HPTF-2, was isolated from a tidal flat in the Yellow Sea in South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain HPTF-2 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain HPTF-2 fell within the clade comprising the type strains of species of the genus . Strain HPTF-2 exhibited 16S rRNA gene sequence similarity values of 97.1–98.0 % to the type strains of , , , , , , and , and of 95.1–96.7 % to the type strains of the other species of the genus . Strain HPTF-2 contained MK-6 as the predominant menaquinone and iso-C 3-OH, iso-C and iso-C G as the major fatty acids. The major polar lipids of strain HPTF-2 were phosphatidylethanolamine and two unidentified lipids. Mean DNA–DNA relatedness values of strain HPTF-2 with the type strains of the nine phylogenetically related species of the genus were 10–26 %. The DNA G+C content of strain HPTF-2 was 38.4 mol%. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain HPTF-2 is separated from other species of the genus . On the basis of the data presented, strain HPTF-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HPTF-2 (=KCTC 52251=NBRC 112297).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001341
2016-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/10/4236.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001341&mimeType=html&fmt=ahah

References

  1. Barbeyron T., Carpentier F., L'haridon S., Schüler M., Michel G., Amann R. 2008; Description of Maribacter forsetii sp. nov., a marine Flavobacteriaceae isolated from North Sea water, and emended description of the genus Maribacter . Int J Syst Evol Microbiol 58:790–797 [View Article][PubMed]
    [Google Scholar]
  2. Barrow G. I., Feltham R. K. A. 1993 Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press; [CrossRef]
    [Google Scholar]
  3. Baumann P., Baumann L. 1981; The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . In The Prokaryotes pp 1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
    [Google Scholar]
  4. Bernardet J.-F. 2011; Family I. Flavobacteriaceae Reichenbach 1992. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 4 , pp. 106–111 Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B. New York: Springer;
    [Google Scholar]
  5. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  6. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868 [View Article][PubMed]
    [Google Scholar]
  7. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [View Article][PubMed]
    [Google Scholar]
  8. Cho K. H., Hong S. G., Cho H. H., Lee Y. K., Chun J., Lee H. K. 2008; Maribacter arcticus sp. nov., isolated from Arctic marine sediment. Int J Syst Evol Microbiol 58:1300–1303 [View Article][PubMed]
    [Google Scholar]
  9. CLSI 2012 Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard—Eleventh Edition CLSI document M02-A11 Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  10. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Comp Physiol 49:25–68 [View Article]
    [Google Scholar]
  11. Embley T. M., Wait R. 1994; Structural lipids of eubacteria. In Chemical Methods in Prokaryotic Systematics , pp. 121–161 . Edited by Goodfellow M., O’Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  12. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  13. Hu J., Yang Q.-Q., Ren Y., Zhang W.-W., Zheng G., Sun C., Pan J., Zhu X.-F., Zhang X.-Q., Wu M. 2015; Maribacter thermophilus sp. nov., isolated from an algal bloom in an intertidal zone, and emended description of the genus Maribacter . Int J Syst Evol Microbiol 65:36–41 [View Article][PubMed]
    [Google Scholar]
  14. Jackson S. A., Kennedy J., Morrissey J. P., O'Gara F., Dobson A. D. W. 2015; Maribacter spongiicola sp. nov. and Maribacter vaceletii sp. nov., isolated from marine sponges, and emended description of the genus Maribacter . Int J Syst Evol Microbiol 65:2097–2103 [View Article][PubMed]
    [Google Scholar]
  15. Jung Y.-T., Lee J.-S., Yoon J.-H. 2014; Maribacter caenipelagi sp. nov., a member of the Flavobacteriaceae isolated from a tidal flat sediment of the Yellow Sea in Korea. Antonie Van Leeuwenhoek 106:733–742 [View Article][PubMed]
    [Google Scholar]
  16. Komagata K., Suzuki K.-I. 1987; Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [CrossRef]
    [Google Scholar]
  17. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [CrossRef]
    [Google Scholar]
  18. Lo N., Jin H. M., Jeon C. O. 2013; Maribacter aestuarii sp. nov., isolated from tidal flat sediment, and an emended description of the genus Maribacter . Int J Syst Evol Microbiol 63:3409–3414 [View Article][PubMed]
    [Google Scholar]
  19. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  20. Nedashkovskaya O. I., Kim S. B., Han S. K., Lysenko A. M., Rohde M., Rhee M.-S., Frolova G. M., Falsen E., Mikhailov V. V., Bae K. S. 2004; Maribacter gen. nov., a new member of the family Flavobacteriaceae, isolated from marine habitats, containing the species Maribacter sedimenticola sp. nov., Maribacter aquivivus sp. nov., Maribacter orientalis sp. nov. and Maribacter ulvicola sp. nov. Int J Syst Evol Microbiol 54:1017–1023 [View Article][PubMed]
    [Google Scholar]
  21. Nedashkovskaya O. I., Vancanneyt M., De Vos P., Kim S. B., Lee M. S., Mikhailov V. V. 2007; Maribacter polysiphoniae sp. nov., isolated from a red alga. Int J Syst Evol Microbiol 57:2840–2843 [View Article][PubMed]
    [Google Scholar]
  22. Nedashkovskaya O. I., Kim S. B., Mikhailov V. V. 2010; Maribacter stanieri sp. nov., a marine bacterium of the family Flavobacteriaceae . Int J Syst Evol Microbiol 60:214–218 [View Article][PubMed]
    [Google Scholar]
  23. Park S., Won S.-M., Kim H., Park D.-S., Yoon J.-H. 2014; Aestuariivita boseongensis gen. nov., sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 64:2969–2974 [View Article][PubMed]
    [Google Scholar]
  24. Park S., Jung Y.-T., Park J.-M., Won S.-M., Yoon J.-H. 2015; Maribacter confluentis sp. nov., isolated from the junction between the ocean and a freshwater spring. Int J Syst Evol Microbiol 65:3079–3085 [View Article][PubMed]
    [Google Scholar]
  25. Parte A. C. 2014; LPSN-list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 42:D613–D616 [View Article][PubMed]
    [Google Scholar]
  26. Reichenbach H. 1992; The order Cytophagales . In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd edn. pp. 3631–3675 . Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  27. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  28. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 44:846–849 [View Article]
    [Google Scholar]
  29. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942[PubMed]
    [Google Scholar]
  30. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  31. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  32. Weerawongwiwat V., Kang H., Jung M. Y., Kim W. 2013; Maribacter chungangensis sp. nov., isolated from a green seaweed, and emended descriptions of the genus Maribacter and Maribacter arcticus . Int J Syst Evol Microbiol 63:2553–2558 [View Article][PubMed]
    [Google Scholar]
  33. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [View Article]
    [Google Scholar]
  34. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [View Article][PubMed]
    [Google Scholar]
  35. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. 2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [View Article][PubMed]
    [Google Scholar]
  36. Yoon J.-H., Kang S.-J., Lee S.-Y., Lee C.-H., Oh T.-K. 2005; Maribacter dokdonensis sp. nov., isolated from sea water off a Korean island, Dokdo. Int J Syst Evol Microbiol 55:2051–2055 [View Article][PubMed]
    [Google Scholar]
  37. Zhang G. I., Hwang C. Y., Kang S.-H., Cho B. C. 2009; Maribacter antarcticus sp. nov., a psychrophilic bacterium isolated from a culture of the Antarctic green alga Pyramimonas gelidicola . Int J Syst Evol Microbiol 59:1455–1459 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001341
Loading
/content/journal/ijsem/10.1099/ijsem.0.001341
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error