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Volume 66, Issue 11

sp. nov., isolated from oil-contaminated soil Free

Abstract

During the study of hydrocarbon-degrading bacteria in the oil-contaminated soil of Gunsan, North Jeolla Province, South Korea, a yellow, Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated strain DKC-5-1, was isolated. This strain was non-sporulating, catalase-negative and oxidase-positive. It was able to grow at 10–33 °C, pH 6.0–8.5 and at an NaCl concentration of 0–1.5 % (w/v). This strain was characterized taxonomically using a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain DKC-5-1 belongs to the genus and is closely related to LNB2 (96.65 % sequence similarity), A175 (96.63 % sequence similarity), UM2 (96.63 % sequence similarity), and RW1 (96.43 % sequence similarity). The only respiratory quinone was ubiquinone-10 and the major polyamine was homospermidine. The polar lipid profile revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and phosphatidyldimethylethanolamine. The predominant fatty acids of strain DKC-5-1 were summed feature 8 (Cω7 and/or Cω6), C, C 2-OH, summed feature 3 (Cω7 and/or Cω6), Cω6 and C. The genomic DNA G+C content of this novel strain was 65.9 mol%. Morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain DKC-5-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DKC-5-1 (=KEMB 9005-380=KACC 18716=JCM 31294).

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Keyword(s): oil-contaminated soil , Proteobacteria , Sphingomonadaceae and Sphingomonas naphthae sp. nov.
© 2016 IUMS
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2016-11-01
2024-04-19
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Sphingomonas naphthae sp. nov., isolated from oil-contaminated soil
Int J Syst Evol Microbiol 66, 4621 (2016); https://doi.org/10.1099/ijsem.0.001400
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