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Volume 66, Issue 11

gen. nov., sp. nov., a new member of the family , isolated from activated sludge Free

Abstract

A novel Gram-stain-negative, non-spore-forming and short rod-shaped bacterium, strain LIP-5, isolated from an activated sludge in a pesticide factory in Xinyi, China, was investigated for its taxonomic position by a polyphasic approach. Cell growth occurred at 16–42 °C (optimum, 30 °C), in the presence of 0–3.0 % (w/v) NaCl (optimum, without NaCl) and at pH 6.0–9.0 (optimum, pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the isolate was a member of the family , with the closest relatives being Vu-144 (93.5 % similarity), followed by L2-4 (90.5 %) and Gsoil 052 (89.6 %). Menaquinone 7 (MK-7) was the predominant respiratory ubiquinone and phosphatidylethanolamine and unidentified lipids were the major polar lipids. The major cellular fatty acids of strain LIP-5 were anteiso-C and iso-C, and there were also moderate amounts of iso-C 3-OH and C 2-OH. The DNA G+C content was 42.3 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain LIP-5 was classified as representing a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is LIP-5 (=CCTCC AB 2015365=KCTC 42956).

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  • Accepted:
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Keyword(s): 16S rRNA gene , Gram-stain negative , Haoranjiania flava gen. nov., sp. nov. and taxonomy
© 2016 IUMS
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2016-11-01
2024-04-25
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Haoranjiania flava gen. nov., sp. nov., a new member of the family Chitinophagaceae, isolated from activated sludge
Int J Syst Evol Microbiol 66, 4686 (2016); https://doi.org/10.1099/ijsem.0.001411
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/content/journal/ijsem/10.1099/ijsem.0.001411
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