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Volume 66, Issue 11

sp. nov., a halotolerant actinobacterium isolated from rhizosphere soil of , and emended description of the genus Free

Abstract

A halotolerant actinobacterium, designated J12GA03, was isolated from a rhizosphere soil sample of collected from a dried saline lake in Hebei Province, China. Cells were Gram-staining-positive, non-motile and non-spore-forming cocci. Strain J12GA03 grew optimally at 28‒37 °C, 0‒3 % NaCl (w/v) and pH 6.5‒7.5. It contained -diaminopimelic acid as the diagnostic diamino acid and arabinose, galactose and ribose as the diagnostic whole-cell sugars. MK-8 and MK-7 were detected as predominant menaquinones. Major fatty acids were C 8, C and C. Polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylglycerol, phosphoglycolipids, glycolipids, unidentified phospholipids and additional lipids. The muramyl residue was acetyl. Mycolic acids (34–38 carbon atoms) were present. The G+C content of the genomic DNA was 55.8 mol%. It shared the highest 16S rRNA gene sequence similarities with DQS3-9A1 (98.18 %) and OFN S31 (97.75 %). Phylogenetic trees showed that strain J12GA03 firmly formed a distinct monophyletic branch in the clade with . DQS3-9A1 and . DSM 45258. The levels of DNA–DNA relatedness with . DSM 45089 and . DSM 45258 were 39.7±3.9 % and 35.7±3.0 %, respectively. Combining the evidence from the polyphasic taxonomic study, strain J12GA03 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is J12GA03 (=DSM 101985=CGMCC 1.15478).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): halotolerant actinobacterium , Hoyosella , Hoyosella rhizosphaerae , saline lake and Suaeda salsa
© 2016 IUMS
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2016-11-01
2024-04-24
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Hoyosella rhizosphaerae sp. nov., a halotolerant actinobacterium isolated from rhizosphere soil of Suaeda salsa, and emended description of the genus Hoyosella
Int J Syst Evol Microbiol 66, 4716 (2016); https://doi.org/10.1099/ijsem.0.001416
/content/journal/ijsem/10.1099/ijsem.0.001416
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