%0 Journal Article %A Hezbri, Karima %A Louati, Moussa %A Nouioui, Imen %A Gtari, Maher %A Rohde, Manfred %A Spröer, Cathrin %A Schumann, Peter %A Klenk, Hans-Peter %A Ghodhbane-Gtari, Faten %A Montero-Calasanz, Maria del Carmen %T Blastococcus capsensis sp. nov., isolated from an archaeological Roman pool and emended description of the genus Blastococcus, B. aggregatus, B. saxobsidens, B. jejuensis and B. endophyticus %D 2016 %J International Journal of Systematic and Evolutionary Microbiology, %V 66 %N 11 %P 4864-4872 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001443 %K GGDC web server %K Microarray phenotype %K polar lipids %I Microbiology Society, %X A novel Gram-reaction-positive actinobacterium, designated BMG 804T, was isolated from an archaeological Roman pool located in Gafsa, Tunisia. The strain grew as dry bright orange colonies at 30 °C and pH 6.0–8.0. It contained meso-diaminopimelic acid in the cell wall. The whole-cell sugars consisted of glucose, rhamnose and ribose. Polar lipids present were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, an unidentified glycolipid and two unidentified phospholipids. MK-9(H4) was the predominant menaquinone. The fatty acid profile contained major amounts (>5 %) of C17 : 1ω8c, C16 : 1ω7c, iso-C15 : 0, iso-C16 : 0 and iso-C16 : 1H. The 16S rRNA gene sequence of BMG 804T showed 99.4 % as highest sequence similarity with Blastococcus saxobsidens . DNA–DNA hybridization between strain BMG 804T and B. saxobsidens DSM 44509T was 48.6±6.6 %. The G+C content of the DNA was 73.7 mol%. On the basis of the phenotypic and genotypic characteristics, including DNA–DNA hybridization results, BMG 804T (=DSM 46835T=CECT 8876T) is proposed as the type strain of a novel species Blastococcus capsensis sp. nov. Emended descriptions of the genus Blastococcus and the species Blastococcus aggregatus , B. saxobsidens , Blastococcus jejuensis and Blastococcus endophyticus are also proposed. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001443