1887

Abstract

In this work we revise the taxonomic status of the -nodulating strains MAFF 303099 and R7A isolated in Japan and New Zealand, respectively. Their 16S rRNA gene sequences are identical and show 98.0, 99.7, 99.8 and 99.9 % similarity values with respect to NZP 2213, ATCC 33669, USDA 4779 (=CCBAU 2609) and USDA 3471, respectively. The analysis of and gene sequeces showed that ATCC 33669 and USDA 4779 (=CCBAU 2609) are the most closely related strains to MAFF 303099 and R7A, with similarity values suggesting that these two strains belong to a different species for which MAFF 303099 is selected as the type strain. The DNA–DNA relatedness values between strain MAFF 303099 and its closest phylogenetic relatives ranged from 53 to 60 % in average. Strains MAFF 303099 and R7A presented slight differences in the proportions of Cω7 11-methyl and C cyclo ω8 fatty acids with respect to ATCC 33669 and USDA 4779, and also in several phenotypic characteristics. Therefore, we propose the reclassification of these two strains into a novel species named sp. nov., with the type strain being MAFF 303099 (=LMG 29417=CECT 9101).

Keyword(s): Lotus , Mesorhizobium and Taxonomy
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2016-12-01
2024-03-28
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