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Paenibacillus oryzae sp. nov., isolated from rice roots

  • Authors: Jun Zhang1​,2​,†,  Xiao-Tong Ma1​,†,  Jun-Sheng Gao1​,3​,  Juan-Juan Zhao1​,  Hua-Qun Yin4​,  Cai-Wen Zhang1​,  Rui-Jie Zhang1​, Xiao-Xia Zhang1​
    • VIEW AFFILIATIONS
    • 1​ 1​Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China 2​ 2​College of Life Science, The Yangtze University, Jingzhou 434025, PR China 3​ 3​Qiyang Agro-ecosystem of National Field Experimental Station, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Qiyang 426182, PR China 4​ 4​School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, PR China
  • Correspondence Xiao-Xia Zhang zhangxiaoxia@caas.cn
  • First Published Online: 01 December 2016, International Journal of Systematic and Evolutionary Microbiology 66: 5000-5004, doi: 10.1099/ijsem.0.001459
  • Subject: New taxa - Firmicutes and related organisms
  • Received:
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  • A novel endophytic bacterium, strain 1DrF-4, isolated from rice roots, was characterized on the basis of its phenotypic characteristics and genotypic information. The novel strain was Gram-positive-staining, endospore-forming, facultatively anaerobic, motile and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 1DrF-4 formed a monophyletic clade within the genus Paenibacillus . The most phylogenetically related species was Paenibacillus pinesoli KACC 17472, with which strain 1DrF-4 showed 16S rRNA gene sequence similarity of 95.2 %. 16S rRNA gene sequence similarities with type strains of other species of the genus Paenibacillus were less than 95 %. The predominant cellular fatty acids were anteiso-C15 : 0 (61.1 %) and C16 : 0 (11.1 %), which is one of the characteristic traits of the genus Paenibacillus . The quinone system contained exclusively menaquinone MK-7. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, glycolipid and an unknown phospholipid. The DNA G+C content was 50.16 mol%, which was within the range reported for species of the genus Paenibacillus . Characterization by genotypic, chemotaxonomic and phenotypic analysis indicated that strain 1DrF-4 (=ACCC 19927=JCM 30486) represents a novel species of the genus Paenibacillus , for which the name Paenibacillus oryzae sp. nov. is proposed.

  • †

    These authors contributed equally to this work.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 1DrF-4 is KX196453.

  • Eight supplementary figures are available with the online Supplementary Material.

© 2016 IUMS | Published by the Microbiology Society

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2017-07-24
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