RT Journal Article SR Electronic(1) A1 Wen, Zhi-Qiang A1 Chen, Bingzhi A1 Li, Xiao A1 Li, Bing-Bing A1 Li, Cheng-Huan A1 Huang, Qing-Hua A1 Zhang, Qi-Hui A1 Dai, Wei-hao A1 Jiang, Yu-JiYR 2016 T1 Streptomyces tremellae sp. nov., isolated from a culture of the mushroom Tremella fuciformis JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 12 SP 5028 OP 5033 DO https://doi.org/10.1099/ijsem.0.001464 PB Microbiology Society, SN 1466-5034, AB A novel actinomycete strain, designated Js-1T, was isolated from Tremella fuciformis collected from Gutian, Fujian Province, in southeastern China. The taxonomic status of this strain was determined by a polyphasic approach, which demonstrated that the novel strain was a member of the genus Streptomyces . The cell walls of this strain were found to contain ll-diaminopimelic acid, muramic acid and glycine. An analysis of whole-cell hydrolysates revealed that no characteristic sugar was present. The key identified menaquinones were MK-9 (H6) and MK-9 (H8), while the diagnostic polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylmethylethanolamine and phosphatidylglycerol. The main cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0, C16 : 0 and iso-C16 : 0. An analysis of an almost complete 16S rRNA gene sequence showed that the strain shared the highest levels of sequence similarity with Streptomyces sannanensis KC-7038T (97.87 %), Streptomyces hebeiensis YIM 001T (97.84 %), Streptomyces pathocidini NBRC 13812T (97.80 %), Streptomyces cocklensis BK168T (97.25 %), Streptomyces coerulescens NBRC 12758T (97.12 %), Streptomyces aurantiogriseus NBRC 12842T (97.06 %) and Streptomyces rimosus subsp. rimosus ATCC 10970T (97.04 %). The DNA G+C content of the genomic DNA of strain Js-1T was 70.1 mol%. Furthermore, DNA–DNA hybridization tests revealed that the relatedness values between strain Js-1T and the most closely related species ranged from 15.10 to 47.20 %. Based on its phenotypic and genotypic characteristics, strain Js-1T (=CCTCC M 2011365T=JCM 30846T) is considered to represent a novel species within the genus Streptomyces , which we classified as Streptomyces tremellae sp. nov., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001464