RT Journal Article SR Electronic(1) A1 Jin, Decai A1 Kong, Xiao A1 Li, Honghong A1 Luo, Luyun A1 Zhuang, Xuliang A1 Zhuang, Guoqiang A1 Deng, Ye A1 Bai, ZhihuiYR 2016 T1 Patulibacter brassicae sp. nov., isolated from rhizosphere soil of Chinese cabbage (Brassica campestris) JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 12 SP 5056 OP 5060 DO https://doi.org/10.1099/ijsem.0.001469 PB Microbiology Society, SN 1466-5034, AB A novel actinobacterial strain, designated SDT, was isolated from rhizosphere soil of Chinese cabbage in Shandong province, China. The cells were aerobic, Gram-staining-positive, oxidase- and catalase-positive, short rods and formed white colonies on trypticase soy agar. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid and alanine, glutamic acid and leucine. Diphosphatidylglycerol was the predominant polar lipid. The predominant cellular fatty acid was C18 : 1 ω9c; minor components were anteiso-C15 : 0 and anteiso-C17 : 0. The only isoprenoid quinone was demethylmenaquinone 7 (DMK-7), and the DNA G+C content was 72.7 mol%. Based on the full-length 16S rRNA gene sequence analysis, the closest phylogenetic neighbours of strain SDT were Patulibacter medicamentivorans DSM 25962T (96.9 %), Patulibacter minatonensis DSM 18081T (96.7 %), Patulibacter americanus DSM 16676T (96.2 %) and Patulibacter ginsengiterrae DSM 25990T (95.9 %). Considering combined phenotypic and genotypic characteristics, it is proposed that strain SDT represents a novel species of the genus Patulibacter , for which the name Patulibacter brassicae sp. nov. is proposed. The type strain is SDT (=CICC 24108T=KCTC 39817T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001469