Novosphingobium bradum sp. nov., isolated from a spring
Sheu, Shih-Yi and Liu, Li-Ping and Chen, Wen-Ming,, 66, 5083-5090 (2016),
doi = https://doi.org/10.1099/ijsem.0.001475,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A bacterial strain designated STM-24T was isolated from a spring in Taiwan and characterized using the polyphasic taxonomic approach. Cells of strain STM-24T were Gram-staining- negative, aerobic, poly-β-hydroxybutyrate accumulating, non-motile, rod shaped and forming light yellow-coloured colonies. Growth occurred at 15–30 °C (optimum, 20 °C), at pH 5.0–9.0 (optimum, pH 6.0) and with 0–0.5 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain STM-24T belonged to the genus Novosphingobium and was most closely related to Novosphingobium piscinae SLH-16T with sequence similarity of 98.4 %. The major fatty acids (>10 %) of strain STM-24T were C18 : 1ω7c, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The major 2-hydroxy fatty acid was C14 : 0 2-OH. The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipid, phosphatidylcholine and several uncharacterized lipids. The major polyamine was spermidine. The major isoprenoid quinone was Q-10. The DNA G+C content was 69.1mol%. The DNA–DNA hybridization value for strain STM-24T with N . piscinae SLH-16T, Novosphingobium taihuense JCM 12465T and Novosphingobium subterraneum DSM 12447T was less than 55 %. Phenotypic characteristics of the novel strain also differed from those of the closest related species of the genus Novosphingobium . On the basis of the genotypic, chemotaxonomic and phenotypic data, strain STM-24T represents a novel species in the genus Novosphingobium , for which the name Novosphingobium bradum sp. nov. is proposed. The type strain is STM-24T (=BCRC 80925T=LMG 29291T=KCTC 42984T).,
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