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Description of ‘Candidatus Phytoplasma meliae’, a phytoplasma associated with Chinaberry (Melia azedarach L.) yellowing in South America

  • Authors: Franco Daniel Fernández1​,  Ernestina Galdeano2​,  Marcela Victoria Kornowski3​,  Joel Demián Arneodo4​, Luis Rogelio Conci1​
    • VIEW AFFILIATIONS
    • 1​ 1​Instituto de Patología Vegetal (IPAVE), CIAP-INTA, Camino 60 cuadras km 5 ½ (X5020ICA), Córdoba, Argentina 2​ 2​Instituto de Botánica del Nordeste, (CONICET-UNNE), Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Sargento Cabral 2131 (3400), Corrientes, Argentina 3​ 3​Estación Experimental Agropecuaria Montecarlo-INTA, Av. El Libertador 2472 (3384), Montecarlo, Argentina 4​ 4​Instituto de Microbiología y Zoología Agrícola (IMyZA), INTA, Nicolas Repetto y de los Reseros s/n (1686), Hurlingham, Argentina
  • Correspondence Luis Rogelio Conci conci.luis@inta.gob.ar
  • First Published Online: 01 December 2016, International Journal of Systematic and Evolutionary Microbiology 66: 5244-5251, doi: 10.1099/ijsem.0.001503
  • Subject: New taxa - other bacteria
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Description of ‘Candidatus Phytoplasma meliae’, a phytoplasma associated with Chinaberry (Melia azedarach L.) yellowing in South America, Page 1 of 1

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  • China tree yellows (ChTY) phytoplasma is associated with the yellowing disease of the China tree (Melia azedarach) in Argentina. According to partial 16S rRNA gene analysis, ChTY phytoplasma belongs to the 16Sr XIII group, subgroup G. Strains of species of ChTY have 98–99 % 16S rDNA gene sequence similarity with 16SrXIII-group phytoplasmas, and less than 97.5 % when compared to all ‘Candidatus Phytoplasma ’ described so far, except for the novel ‘Candidatus Phytoplasma hispanicum '. However, strains of species of ChTY are differentiated from the latter due to having additional molecular and biological attributes. The presence of unique features in the 16S rDNA sequence distinguishes ChTY from all species of ‘Candidatus Phytoplasma ’ currently described. The in silico RFLP profile of 16S rDNA (1.2 kb) and rpLV-rpsC (1.3 kb) genes distinguished ChTY, as in the 16SrXIII-G subgroup within the 16SrXIIII group. The phylogenetic analyses, based on 16S rDNA, rpLV-rpsC and secA gene sequences, in addition to the restricted host range, characteristic symptoms and geographical distribution, confirm that the collective strains of the species ChTY represent a distinct lineage within the phytoplasma clade and support the description of a novel species of ‘Candidatus Phytoplasma meliae ’ with the reference strain being ChTY-Mo3 (Montecarlo, Argentina).

  • GenBank/EMBL/DDBJ accession numbers of gene sequence from representative strain [‘Ca. Phytoplasma meliae’ ChTY-Mo3 are: KU850940, KU850944 and KU850948 (16S rDNA, secA and rpLV-rpsC genes respectively)].

  • Six supplementary figures and one supplementary table are available with the online Supplementary Material.

  • Abbreviations:
    ChTY China tree yellows
    MPV Mexican periwinkle virescence phytoplasma
    RFLP Restriction Fragment Length Polymorphism
    SNP Single Nucleotide Polymorphism

© 2016 IUMS | Published by the Microbiology Society

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2017-07-18
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