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Volume 66, Issue 12

sp. nov., a moderately halophilic, endophytic bacterium isolated from a poplar tree, and emended description of the genus Free

Abstract

A Gram-staining-positive, moderately halophilic, aerobic, endospore-forming, rod-shaped bacterial strain, designated WD4L-1 was isolated from surface-sterilized stem tissue of a poplar tree planted in the Wudalianchi National Geopark of Heilongjiang province, PR China. This novel isolate grew in the presence of 0–15 % (w/v) NaCl, at pH 6.0–9.0 and 15–50 °C; optimum growth was observed with 7–8 % (w/v) NaCl, at pH 7.0 and 30 °C. The 16S rRNA gene sequence analysis indicated that the strain WD4L-1 belonged to the genus , and was most closely related to SL-MJ1 with a sequence similarity of 96.1 %. The DNA G+C content of strain WD4L-1 was determined to be 36.9 mol%. The respiratory quinone was identified as menaquinone-7 (MK-7) and the major lipids were diphosphatidylglycerol and phosphatidylglycerol and one unidentified phospholipid. The major fatty acids of strain WD4L-1 were anteiso-C, iso-C and anteiso-C. The results of the physiological and biochemical tests and the minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain WD4L-1 from the closely related JCM 30131. Thus, strain WD4L-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WD4L-1 (=CGMCC 1.15454=DSM 101738). An emended description of the genus is also provided.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): endophytic bacterium , Lentibacillus populi and poplar tree.
© 2016 IUMS
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2016-12-01
2024-04-25
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Lentibacillus populi sp. nov., a moderately halophilic, endophytic bacterium isolated from a poplar tree, and emended description of the genus Lentibacillus
Int J Syst Evol Microbiol 66, 5281 (2016); https://doi.org/10.1099/ijsem.0.001508
/content/journal/ijsem/10.1099/ijsem.0.001508
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