RT Journal Article SR Electronic(1) A1 Sun, Pengbo A1 Gao, Jun-lian A1 Mao, Xiao-jie A1 Zhao, Xing-hua A1 Sun, Jian-guang A1 Lu, MengzhuYR 2016 T1 Lentibacillus populi sp. nov., a moderately halophilic, endophytic bacterium isolated from a poplar tree, and emended description of the genus Lentibacillus JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 12 SP 5281 OP 5287 DO https://doi.org/10.1099/ijsem.0.001508 PB Microbiology Society, SN 1466-5034, AB A Gram-staining-positive, moderately halophilic, aerobic, endospore-forming, rod-shaped bacterial strain, designated WD4L-1T was isolated from surface-sterilized stem tissue of a poplar tree planted in the Wudalianchi National Geopark of Heilongjiang province, PR China. This novel isolate grew in the presence of 0–15 % (w/v) NaCl, at pH 6.0–9.0 and 15–50 °C; optimum growth was observed with 7–8 % (w/v) NaCl, at pH 7.0 and 30 °C. The 16S rRNA gene sequence analysis indicated that the strain WD4L-1T belonged to the genus Lentibacillus , and was most closely related to Lentibacillus garicola SL-MJ1T with a sequence similarity of 96.1 %. The DNA G+C content of strain WD4L-1T was determined to be 36.9 mol%. The respiratory quinone was identified as menaquinone-7 (MK-7) and the major lipids were diphosphatidylglycerol and phosphatidylglycerol and one unidentified phospholipid. The major fatty acids of strain WD4L-1T were anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0. The results of the physiological and biochemical tests and the minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain WD4L-1T from the closely related Lentibacillus garicola JCM 30131T. Thus, strain WD4L-1T represents a novel species of the genus Lentibacillus , for which the name Lentibacillus populi sp. nov. is proposed. The type strain is WD4L-1T (=CGMCC 1.15454T=DSM 101738T). An emended description of the genus Lentibacillus is also provided., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001508