Corynebacterium crudilactis sp. nov., isolated from raw cow's milk
Zimmermann, Julia and Rückert, Christian and Kalinowski, Jörn and Lipski, André,, 66, 5288-5293 (2016),
doi = https://doi.org/10.1099/ijsem.0.001509,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A Gram-stain-positive, rod-shaped bacterium (strain JZ16T) was isolated from raw cow's milk from the bulk tank of a dairy farm in Germany. The 16S rRNA gene sequence of the isolate showed a similarity of 98.3 % to the nearest related type strain Corynebacterium glutamicum ATCC 13032T, a similarity of 97.6 % to Corynebacterium deserti GIMN1.010T and a similarity of 97.4 % to Corynebacterium callunae DSM 20147T. Determination of chemotaxonomic characteristics revealed oleic acid (18 : 1 cis 9) as the predominant fatty acid, major amounts of hexadecanoic acid (16 : 0) and minor amounts of heptadecanoic acid (17 : 0). The isolate showed an acetyl type of peptidoglycan and corynemycolic acids. The menaquinones MK-8(H2) and MK-9(H2) and the phospholipids diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected, which was in agreement with the description of the genus Corynebacterium . Strain JZ16T was positive for reduction of nitrate to nitrite, pyrazinamidase, β-glucuronidase, β-glucosidase and urease activities. Acid was produced from d-glucose, d-ribose and d-mannitol, but not from d-xylose, maltose, lactose, sucrose and glycogen. The results of phylogenetic, phenotypic and chemotaxonomic analyses enabled the differentiation of the isolated strain from other closely related species of the genus Corynebacterium . Therefore, strain JZ16T represents a novel species of the genus Corynebacterium , for which the name Corynebacterium crudilactis sp. nov. is proposed. The type strain is JZ16T (=DSM 100882T=CCUG 69192T=LMG 29813T).,
language=,
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