@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001515, author = "Kang, Jong-Pyo and Kim, Yeon-Ju and Nguyen, Ngoc-Lan and Hoang, Van-An and Farh, Mohamed El-Agamy and Joo, Sung-Chul and Quan, Lin-Hu and Yang, Deok-Chun", title = "Phycicoccus ginsengisoli sp. nov., isolated from cultivated ginseng soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "12", pages = "5320-5327", doi = "https://doi.org/10.1099/ijsem.0.001515", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001515", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Taxonomy", keywords = "Actinobacteria", keywords = "Phycicoccus ginsengisoli", keywords = "ginseng soil", abstract = "Ginseng-cultivated soil is an excellent habitat for soil-borne bacteria to proliferate. A novel strain, DCY87T, was isolated from ginseng-cultivated soil in Gochang County, Republic of Korea, and subsequently characterized by polyphasic approach. Cells were rod shaped, non-motile, aerobic, Gram-reaction-positive, oxidase-negative and catalase-positive. 16S rRNA gene sequence analysis showed that strain DCY87T shared the highest similarity to ‘ Phycicoccus ochangensis ’ L1b-b9 (98.7 %). Closely phylogenetic relatives of strain DCY87T were identified: Phycicoccus ginsenosidimutans BXN5-13T (97.9 %), Phycicoccus soli THG-a14T (97.8 %), Phycicoccus bigeumensis MSL-03T (97.3 %), Phycicoccus cremeus V2M29T (97.3 %), Phycicoccus aerophilus 5516T-20T (97.3 %), Phycicoccus dokdonensis DS-8T (97.3 %) and Phycicoccus jejuensis KSW2-15T (97.1 %). The major polar lipids were classified as phosphatidylinositol and diphosphatidylglycerol. The major cellular fatty acids were composed of iso-C15 : 0, anteiso-C15:0, C17 : 0 and C17 : 1 ω8c. The menaquinone was resolved as MK-8(H4). Strain DCY87T contained meso-diaminopimelic acid as diamino acid in the cell-wall peptidoglycan and glucose, xylose and rhamnose in the whole-cell sugar. The genomic DNA G+C content was calculated to be 72.7 mol%. DNA–DNA hybridization value between strain DCY87T and ‘ P. ochangensis ’ L1b-b9 was estimated to be 50 %. However, DNA–DNA hybridization value obtained between strain DCY87T and P. ginsenosidimutans BXN5-13T, P. soli THG-a14T and P. bigeumensis MSL-03T was well below 17 %. In general, polyphasic taxonomy demonstrated that DCY87T strain represented a novel species within the genus Phycicoccus . Accordingly, we propose the name Phycicoccus ginsengisoli sp. nov. The type strain is DCY87T (=KCTC 39635T=JCM 31016T).", }