@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001519, author = "Makk, Judit and Tóth, Erika M. and Anda, Dóra and Pál, Sára and Schumann, Peter and Kovács, Attila L. and Mádl-Szőnyi, Judit and Márialigeti, Károly and Borsodi, Andrea K.", title = "Deinococcus budaensis sp. nov., a mesophilic species isolated from a biofilm sample of a hydrothermal spring cave", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "12", pages = "5345-5351", doi = "https://doi.org/10.1099/ijsem.0.001519", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001519", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Deinococcus", keywords = "hydrothermal spring cave", abstract = "Following the exposure of a biofilm sample from a hydrothermal spring cave (Gellért Hill, Budapest, Hungary) to gamma radiation, a strain designated FeSTC15-38T was isolated and studied by polyphasic taxonomic methods. The spherical-shaped cells stained Gram-negative, and were aerobic and non-motile. The pH range for growth was pH 6.0–9.0, with an optimum at pH 7.0. The temperature range for growth was 20–37 °C, with an optimum at 28 °C. Phylogenetic analysis based on the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus Deinococcus. The highest sequence similarities appeared with Deinococcus hopiensis KR-140T (94.1 %), Deinococcus aquaticus PB314T (93.3 %) and Deinococcus aerophilus 5516T-11T (92.7 %). The DNA G+C content of the novel strain was 68.2 mol%. The predominant fatty acids (>10 %) were iso-C16 : 0 and C16 : 1ω7c, and the cell-wall peptidoglycan type was A3β l-Orn–Gly2-3, corroborating the assignment of the strain to the genus Deinococcus. Strain FeSTC15-38T contained MK-8 as the major menaquinone and several unidentified phospholipids, glycolipids and phosphoglycolipids. Resistance to gamma radiation (D10) of strain FeSTC15-38T was <3.0 kGy. According to phenotypic and genotypic data, strain FeSTC15-38T represents a novel species for which the name Deinococcus budaensis sp. nov. is proposed. The type strain is FeSTC15-38T (=NCAIM B.02630T=DSM 101791T).", }