@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001520, author = "Liu, Chongxi and Ye, Lan and Li, Yao and Jiang, Shanwen and Liu, Hui and Yan, Kai and Xiang, Wensheng and Wang, Xiangjing", title = "Streptomyces kronopolitis sp. nov., an actinomycete that produces phoslactomycins isolated from a millipede (Kronopolites svenhedind Verhoeff)", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "12", pages = "5352-5357", doi = "https://doi.org/10.1099/ijsem.0.001520", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001520", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Streptomyces kronopolitis sp. nov.,", keywords = "16S rRNA gene", keywords = "Kronopolites svenhedind Verhoeff", keywords = "Polyphasic taxonomy", keywords = "phoslactomycins", abstract = "A phoslactomycin-producing actinomycete, designated strain NEAU-ML8T, was isolated from a millipede (Kronopolites svenhedind Verhoeff) and characterized using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain NEAU-ML8T belongs to the genus Streptomyces with the highest sequence similarities to Streptomyces lydicus NBRC 13058T (99.39 %) and Streptomyces chattanoogensis DSM 40002T (99.25 %). The maximum-likelihood phylogenetic tree based on 16S rRNA gene sequences showed that the isolate formed a distinct phyletic line with NBRC 13058T and S. chattanoogensis DSM 40002T. This branching pattern was also supported by the tree rconstructed with the neighbour-joining method. A combination of DNA–DNA hybridization experiments and phenotypic tests were carried out between strain NEAU-ML8T and its phylogenetically closely related strains, which further clarified their relatedness and demonstrated that NEAU-ML8T could be distinguished from NBRC 13058T and S. chattanoogensis DSM 40002T. Therefore, it is concluded that strain NEAU-ML8T can be classified as representing a novel species of the genus Streptomyces , for which the name Streptomyces kronopolitis sp. nov. is proposed. The type strain is NEAU-ML8T (=DSM 101986T=CGMCC 4.7323T).", }