%0 Journal Article %A Liu, Chongxi %A Ye, Lan %A Li, Yao %A Jiang, Shanwen %A Liu, Hui %A Yan, Kai %A Xiang, Wensheng %A Wang, Xiangjing %T Streptomyces kronopolitis sp. nov., an actinomycete that produces phoslactomycins isolated from a millipede (Kronopolites svenhedind Verhoeff) %D 2016 %J International Journal of Systematic and Evolutionary Microbiology, %V 66 %N 12 %P 5352-5357 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001520 %K Streptomyces kronopolitis sp. nov., %K 16S rRNA gene %K Kronopolites svenhedind Verhoeff %K Polyphasic taxonomy %K phoslactomycins %I Microbiology Society, %X A phoslactomycin-producing actinomycete, designated strain NEAU-ML8T, was isolated from a millipede (Kronopolites svenhedind Verhoeff) and characterized using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain NEAU-ML8T belongs to the genus Streptomyces with the highest sequence similarities to Streptomyces lydicus NBRC 13058T (99.39 %) and Streptomyces chattanoogensis DSM 40002T (99.25 %). The maximum-likelihood phylogenetic tree based on 16S rRNA gene sequences showed that the isolate formed a distinct phyletic line with NBRC 13058T and S. chattanoogensis DSM 40002T. This branching pattern was also supported by the tree rconstructed with the neighbour-joining method. A combination of DNA–DNA hybridization experiments and phenotypic tests were carried out between strain NEAU-ML8T and its phylogenetically closely related strains, which further clarified their relatedness and demonstrated that NEAU-ML8T could be distinguished from NBRC 13058T and S. chattanoogensis DSM 40002T. Therefore, it is concluded that strain NEAU-ML8T can be classified as representing a novel species of the genus Streptomyces , for which the name Streptomyces kronopolitis sp. nov. is proposed. The type strain is NEAU-ML8T (=DSM 101986T=CGMCC 4.7323T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001520