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Volume 66, Issue 12

gen. nov., sp. nov., a thermoactinomycete isolated from superficial sediment of a solar saltern Free

Abstract

A novel filamentous, halophilic, thermotolerant bacterium, strain SMBg3 was isolated from superficial sediment of a solar saltern in Sfax, Tunisia. The isolate is Gram-staining-positive, aerobic, catalase- and oxidase-positive. Optimum growth occurred at 40–45 °C, with 10 % (w/v) NaCl and at pH 8.0–9.0. Long and well developed aerial and substrate mycelia, with long chains of fluorescent and circular spores, were observed on all tested media. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SMBg3 belongs to an independent phylogenetic lineage of the family and shows a gene sequence similarity of 94 % with DSM 45169 94.2 % with DSM 45196, 94.3 % with KCTC 29149, 94.3 % with DSM 45474 and 94.5 % with CECT 8506. The predominant menaquinone is MK-7, but MK-8 and some minor unidentified components are also present in trace amounts. The major cellular fatty acids are anteiso-C, iso-C and iso-C. In addition to four major polar lipids identified as phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylmethylethanolamine, five minor unknown lipids were detected in cell membranes. The DNA G+C content of strain SMBg3 is 51.2 mol%. Strain SMBg3 is distinct from recognized genera of the family by morphological, biochemical and chemotaxonomic characteristics. On the basis of physiological and phylogenetic data, strain SMBg3 represents a novel species of a new genus in the family for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is SMBg3 (=DSM 102817=CCUG 68698).

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Keyword(s): halophilic actinomycete , Paludifilum halophilum gen. nov., sp. nov , polyphasic taxonomy and solar saltern
© 2016 IUMS
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2016-12-01
2024-04-18
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Paludifilum halophilum gen. nov., sp. nov., a thermoactinomycete isolated from superficial sediment of a solar saltern
Int J Syst Evol Microbiol 66, 5371 (2016); https://doi.org/10.1099/ijsem.0.001523
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