1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 66, Issue 12

sp. nov., isolated from estuarine surface water Free

Abstract

A tyrosine-metabolizing, Gram-stain-negative, strictly aerobic, non-spore-forming, curved-rod-shaped, motile (due to monopolar flagellum) marine bacterium, designated strain CC-PW-9, was isolated from estuarine water off Pingtung, Taiwan. Strain CC-PW-9 not only shared highest 16S rRNA gene sequence similarities with representatives (96.4–93.4 %, =26), but also formed a distinct phyletic lineage and coherent phylogenetic cluster associated with those species. Cells of strain CC-PW-9 grew with 6–12 % (w/v) NaCl, at 20–40 °C and at pH 6–9. It produced predominant amounts of phosphatidylethanolamine, plus diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, two unidentified phospholipids, an unidentified phosphoglycolipid, two unidentified lipids and an unidentified aminolipid in moderate to trace amounts. Fatty acids such as iso-C, iso-C and iso-C 9 andor C 10-methyl (summed feature 9) were found in major amounts. The DNA G+C content was 51.1 mol%. Ubiquinone-8 (Q-8) was the sole respiratory quinone. Based on evidence from this polyphasic taxonomic study, strain CC-PW-9 is considered to represent a novel species of the genus , affiliated to the family , for which the name sp. nov. is proposed. The type strain is CC-PW-9 (=JCM 19757=BCRC 80745).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): CC-PW-9 , curved morphology , Marine bacterium , phosphatidylserine and tyrosine-metabolizing
© 2016 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001525
2016-12-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/5384.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001525&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [View Article][PubMed]
     [Google Scholar]
  2. Barrow G. I., Feltham R. K. A. (editors) 1993 Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press; [CrossRef]
     [Google Scholar]
  3. Brettar I., Christen R., Höfle M. G. 2003; Idiomarina baltica sp. nov., a marine bacterium with a high optimum growth temperature isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 53:407–413 [View Article][PubMed]
     [Google Scholar]
  4. Chen M.-H., Sheu S.-Y., Chen C. A., Wang J.-T., Chen W.-M. 2012; Idiomarina aquimaris sp. nov., isolated from the reef-building coral Isopora palifera . Int J Syst Evol Microbiol 62:1536–1542 [View Article][PubMed]
     [Google Scholar]
  5. Choi D. H., Cho B. C. 2005; Idiomarina seosinensis sp. nov., isolated from hypersaline water of a solar saltern in Korea. Int J Syst Evol Microbiol 55:379–383 [View Article][PubMed]
     [Google Scholar]
  6. Collins M. D. 1985; Analysis of isoprenoid quinones. Methods Microbiol 18:329–366 [CrossRef]
     [Google Scholar]
  7. Donachie S. P., Hou S., Gregory T. S., Malahoff A., Alam M. 2003; Idiomarina loihiensis sp. nov., a halophilic gamma-Proteobacterium from the Lō'ihi submarine volcano, Hawai'i. Int J Syst Evol Microbiol 53:1873–1879 [View Article][PubMed]
     [Google Scholar]
  8. Embley T. M., Wait R. 1994; Structural lipids of eubacteria. In Chemical Methods in Prokaryotic Systematics pp. 121–161 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
     [Google Scholar]
  9. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
     [Google Scholar]
  10. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
     [Google Scholar]
  11. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Biol 20:406–416 [View Article]
     [Google Scholar]
  12. GCG 1995 Wisconsin Package Version 8.1 Program Manual Madison, WI: Computer Group;
     [Google Scholar]
  13. Heiner C. R., Hunkapiller K. L., Chen S. M., Glass J., Chen E. Y. 1998; Sequencing multimegabase-template DNA with BigDye terminator chemistry. Genome Res 8:557–561[PubMed]
     [Google Scholar]
  14. Hu Z.-Y., Li Y. 2007; Pseudidiomarina sediminum sp. nov., a marine bacterium isolated from coastal sediments of Luoyuan Bay in China. Int J Syst Evol Microbiol 57:2572–2577 [View Article][PubMed]
     [Google Scholar]
  15. Ivanova E. P., Romanenko L. A., Chun J., Matte M. H., Matte G. R., Mikhailov V. V., Svetashev V. I., Huq A., Maugel T., Colwell R. R. 2000; Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobellii sp. nov. Int J Syst Evol Microbiol 50:901–907 [View Article][PubMed]
     [Google Scholar]
  16. Jean W. D., Shieh W. Y., Chiu H. H. 2006; Pseudidiomarina taiwanensis gen. nov., sp. nov., a marine bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the family Idiomarinaceae . Int J Syst Evol Microbiol 56:899–905 [View Article][PubMed]
     [Google Scholar]
  17. Jean W. D., Leu T. Y., Lee C. Y., Chu T. J., Lin S. Y., Shieh W. Y. 2009; Pseudidiomarina marina sp. nov. and Pseudidiomarina tainanensis sp. nov. and reclassification of Idiomarina homiensis and Idiomarina salinarum as Pseudidiomarina homiensis comb. nov. and Pseudidiomarina salinarum comb. nov., respectively. Int J Syst Evol Microbiol 59:53–59 [View Article][PubMed]
     [Google Scholar]
  18. José León M., Martínez-Checa F., Ventosa A., Sánchez-Porro C. 2015; Idiomarina aquatica sp. nov., a moderately halophilic bacterium isolated from salterns. Int J Syst Evol Microbiol 65:4595–4600 [View Article]
     [Google Scholar]
  19. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [View Article]
     [Google Scholar]
  20. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
     [Google Scholar]
  21. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
     [Google Scholar]
  22. Kwon S.-W., Kim B.-Y., Weon H.-Y., Baek Y.-K., Koo B.-S., Go S.-J. 2006; Idiomarina homiensis sp. nov., isolated from seashore sand in Korea. Int J Syst Evol Microbiol 56:2229–2233 [View Article][PubMed]
     [Google Scholar]
  23. Lee J.-C., Kim Y.-S., Yun B.-S., Whang K.-S. 2015; Idiomarina halophila sp. nov., isolated from a solar saltern sediment. Int J Syst Evol Microbiol 65:1268–1273 [View Article][PubMed]
     [Google Scholar]
  24. Martínez-Cánovas M. J., Béjar V., Martínez-Checa F., Páez R., Quesada E. 2004; Idiomarina fontislapidosi sp. nov. and Idiomarina ramblicola sp. nov., isolated from inland hypersaline habitats in Spain. Int J Syst Evol Microbiol 54:1793–1797 [View Article][PubMed]
     [Google Scholar]
  25. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
     [Google Scholar]
  26. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  27. Montero-Calasanz M. D., Göker M., Rohde M., Spröer C., Schumann P., Busse H.-J., Schmid M., Tindall B. J., Klenk H.-P., Camacho M. 2013; Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery, and emendation of the species Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium . Int J Syst Evol Microbiol 63:4386–4395 [View Article][PubMed]
     [Google Scholar]
  28. Murray R. G. E., Doetsch R. N., Robinow C. F. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp. 21–41 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society of Microbiology;
     [Google Scholar]
  29. Park S. C., Lim C. H., Baik K. S., Lee K. H., Lee J.-S., Seong C. N. 2010; Pseudidiomarina aestuarii sp. nov., a marine bacterium isolated from shallow coastal seawater. Int J Syst Evol Microbiol 60:2071–2075 [View Article][PubMed]
     [Google Scholar]
  30. Poddar A., Lepcha R. T., Mukherjee D., Bhattacharyya D., Das S. K. 2014; Comparative analysis of 16S rRNA signature sequences of the genus Idiomarina and Idiomarina woesei sp. nov., a novel marine bacterium isolated from the Andaman Sea. Res Microbiol 165:501–507 [View Article][PubMed]
     [Google Scholar]
  31. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  32. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:16
     [Google Scholar]
  33. Shahina M., Hameed A., Lin S.-Y., Hsu Y.-H., Liu Y.-C., Cheng I.-C., Lee M.-R., Lai W.-A., Lee R.-J., Young C.-C. 2013; Sphingomicrobium astaxanthinifaciens sp. nov., an astaxanthin-producing glycolipid-rich bacterium isolated from surface seawater and emended description of the genus Sphingomicrobium . Int J Syst Evol Microbiol 63:3415–3422 [View Article][PubMed]
     [Google Scholar]
  34. Sitdhipol J., Visessanguan W., Benjakul S., Yukphan P., Tanasupawat S. 2013; Idiomarina piscisalsi sp. nov., from fermented fish (pla-ra) in Thailand. J Gen Appl Microbiol 59:385–391 [View Article][PubMed]
     [Google Scholar]
  35. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  36. Song L., Ren F., Huang Y., Dai X., Zhou Y. 2013; Idiomarina indica sp. nov., isolated from seawater. Int J Syst Evol Microbiol 63:2497–2500 [View Article][PubMed]
     [Google Scholar]
  37. Taborda M., Antunes A., Tiago I., Veríssimo A., Nobre M. F., da Costa M. S. 2009; Description of Idiomarina insulisalsae sp. nov., isolated from the soil of a sea salt evaporation pond, proposal to transfer the species of the genus Pseudidiomarina to the genus Idiomarina and emended description of the genus Idiomarina . Syst Appl Microbiol 32:371–378 [View Article][PubMed]
     [Google Scholar]
  38. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
     [Google Scholar]
  39. Teather R. M., Wood P. J. 1982; Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 43:777–780[PubMed]
     [Google Scholar]
  40. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  41. Tindall B. J., Rosselló-Móra R., Busse H.-J., Ludwig W., Kämpfer P. 2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266 [View Article][PubMed]
     [Google Scholar]
  42. Wang L., Lai Q., Fu Y., Chen H., Wang W., Wang J., Sun F., Shao Z. 2011; Idiomarina xiamenensis sp. nov., isolated from surface seawatgenus Idiomarina as Idiomarina aestuarii comb. nover, and proposal to transfer Pseudidiomarina aestuarii to the genus Idiomarina as Idiomarina aestuarii comb. nov. Int J Syst Evol Microbiol 61:969–973 [CrossRef]
     [Google Scholar]
  43. Watts D., MacBeath J. R. 2001; Automated fluorescent DNA sequencing on the ABI PRISM 310 genetic Analyzer. Methods Mol Biol 167:153–170 [View Article][PubMed]
     [Google Scholar]
  44. Wu Y.-H., Shen Y.-Q., Xu X.-W., Wang C.-S., Oren A., Wu M. 2009; Pseudidiomarina donghaiensis sp. nov. and Pseudidiomarina maritima sp. nov., isolated from the East China Sea. Int J Syst Evol Microbiol 59:1321–1325 [View Article][PubMed]
     [Google Scholar]
  45. Yoon J.-H., Jung S.-Y., Jung Y.-T., Oh T.-K. 2007; Idiomarina salinarum sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 57:2503–2506 [View Article][PubMed]
     [Google Scholar]
  46. Zhong Z.-P., Liu Y., Liu H.-C., Wang F., Song L., Liu Z.-P. 2014; Idiomarina planktonica sp. nov., isolated from a saline lake. Int J Syst Evol Microbiol 64:3411–3416 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001525
Loading
Idiomarina tyrosinivorans sp. nov., isolated from estuarine surface water
Int J Syst Evol Microbiol 66, 5384 (2016); https://doi.org/10.1099/ijsem.0.001525
/content/journal/ijsem/10.1099/ijsem.0.001525
/content/journal/ijsem/10.1099/ijsem.0.001525
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error