1887

Abstract

Two Gram-stain-negative, obligately aerobic, non-motile, rod-shaped bacterial strains, designated SP3 and SP38, were isolated from a cold-water lake in the west of China. A polyphasic taxonomic study was performed for the strains. Alignment of 16S rRNA gene sequences indicated that strains SP3 and SP38 were associated with the genus and were most closely related to NP180 (96.4 % sequence similarity), GSW-R14 (95.6 %) and hg (95.3 %). The genomic DNA G+C contents of strains SP3 and SP38 were 34.9 and 34.6 mol%, respectively. The major fatty acids were iso-C, iso-C G, summed feature 9 (iso-C 9 and/or 10-methyl C), iso-C 3-OH and iso-C 3-OH. The unique respiratory quinone was menaquinone 6 (MK-6). The polar lipid profile consisted of phosphatidylethanolamine, one unidentified aminolipid and several unidentified polar lipids. Based on physiological, biochemical and phylogenetic data for these isolates, it was confirmed that strains SP3 and SP38 were affiliated to the genus and represented a novel species, for which the name sp. nov. is proposed. The type strain is SP3 (=CGMCC 1.12506=NBRC 109717).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001544
2017-01-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/1/108.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001544&mimeType=html&fmt=ahah

References

  1. Bergey DH, Harrison FC, Breeds RS, Hammer BW, Huntoon FM. (editors) Genus II. Flavobacterium gen. nov. Bergey’s Manual of Determinative Bacteriology Baltimore: Williams & Wilkins; 192397–117
    [Google Scholar]
  2. Feng H, Zeng Y, Huang Y. Flavobacterium palustre sp. nov., isolated from wetland soil. Int J Syst Evol Microbiol 2015; 65:1003–1007 [View Article][PubMed]
    [Google Scholar]
  3. Feng Q, Gao Y, Nogi Y, Tan X, Han L et al. Flavobacterium maotaiense sp. nov., isolated from freshwater. Int J Syst Evol Microbiol 2015; 65:171–176 [View Article][PubMed]
    [Google Scholar]
  4. Gao JL, Lv FY, Wang XM, Yuan M, Li JW et al. Flavobacterium endophyticum sp. nov., a nifH gene-harbouring endophytic bacterium isolated from maize root. Int J Syst Evol Microbiol 2015; 65:3900–3904 [View Article][PubMed]
    [Google Scholar]
  5. Ngo HT, Kook M, Yi TH. Flavobacterium daemonensis sp. nov., isolated from Daemo Mountain soil. Int J Syst Evol Microbiol 2015; 65:983–989 [View Article][PubMed]
    [Google Scholar]
  6. Feng Q, Han L, Yuan X, Tan X, Gao Y et al. Flavobacterium procerum sp. nov., isolated from freshwater. Int J Syst Evol Microbiol 2015; 65:2702–2708 [View Article][PubMed]
    [Google Scholar]
  7. Zhang H, Cheng MG, Sun B, Guo SH, Song M et al. Flavobacterium suzhouense sp. nov., isolated from farmland river sludge. Int J Syst Evol Microbiol 2015; 65:370–374 [View Article][PubMed]
    [Google Scholar]
  8. Zhang Y, Jiang F, Chang X, Qiu X, Ren L et al. Flavobacterium collinsense sp. nov., isolated from a till sample of an Antarctic glacier. Int J Syst Evol Microbiol 2016; 66:172–177 [View Article][PubMed]
    [Google Scholar]
  9. Kämpfer P, Busse HJ, Mcinroy JA, Xu J, Glaeser SP. Flavobacterium nitrogenifigens sp. nov., isolated from switchgrass (Panicum virgatum). Int J Syst Evol Microbiol 2015; 65:2803–2809 [View Article][PubMed]
    [Google Scholar]
  10. Yi H, Oh HM, Lee JH, Kim SJ, Chun J. Flavobacterium antarcticum sp. nov., a novel psychrotolerant bacterium isolated from the Antarctic. Int J Syst Evol Microbiol 2005; 55:637–641 [View Article][PubMed]
    [Google Scholar]
  11. Subhash Y, Sasikala C-H, Ramana C-V. Flavobacterium aquaticum sp. nov., isolated from a water sample of a rice field. Int J Syst Evol Microbiol 2013; 63:3463–3469 [View Article][PubMed]
    [Google Scholar]
  12. Li A, Liu H, Sun B, Zhou Y, Xin Y. Flavobacterium lacus sp. nov., isolated from a high-altitude lake, and emended description of Flavobacterium filum. Int J Syst Evol Microbiol 2014; 64:933–939 [View Article][PubMed]
    [Google Scholar]
  13. Ao L, Zeng XC, Nie Y, Mu Y, Zhou L et al. Flavobacterium arsenatis sp. nov., a novel arsenic-resistant bacterium from high-arsenic sediment. Int J Syst Evol Microbiol 2014; 64:3369–3374 [View Article][PubMed]
    [Google Scholar]
  14. Fu Y, Tang X, Lai Q, Zhang C, Zhong H et al. Flavobacterium beibuense sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 2011; 61:205–209 [View Article][PubMed]
    [Google Scholar]
  15. Kämpfer P, Lodders N, Martin K, Avendaño-Herrera R. Flavobacterium chilense sp. nov. and Flavobacterium araucananum sp. nov., isolated from farmed salmonid fish. Int J Syst Evol Microbiol 2012; 62:1402–1408 [View Article][PubMed]
    [Google Scholar]
  16. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991 pp 115–175
    [Google Scholar]
  17. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  18. Larkin MA, Blackshields G, Brown NP, Chenna R, Mcgettigan PA et al. CLUSTAL W and CLUSTAL X version 2.0. Bioinformatics 2007; 23:2947–2948 [View Article][PubMed]
    [Google Scholar]
  19. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  20. Kishino H, Hasegawa M. Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in hominoidea. J Mol Evol 1989; 29:170–179 [View Article][PubMed]
    [Google Scholar]
  21. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
    [Google Scholar]
  22. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
    [Google Scholar]
  23. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  24. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  25. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp 607–654
    [Google Scholar]
  26. Reichenbach H. The order Cytophagales. In Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH. (editors) The Prokaryotes, 2nd ed. vol. 4 New York: Springer; 1992 pp 3631–3675 [CrossRef]
    [Google Scholar]
  27. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of Deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
    [Google Scholar]
  28. Kuo I, Saw J, Kapan DD, Christensen S, Kaneshiro KY et al. Flavobacterium akiainvivens sp. nov., from decaying wood of Wikstroemia oahuensis, Hawai'i, and emended description of the genus Flavobacterium. Int J Syst Evol Microbiol 2013; 63:3280–3286 [View Article][PubMed]
    [Google Scholar]
  29. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  30. Collins MD. Isoprenoid quinone analysis in classification and identification In Goodfellow M, Minnikin DE. (editors) Chemical Methods in Bacterial Systematics London: Academic Press; 1985 pp. 267–287
    [Google Scholar]
  31. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001544
Loading
/content/journal/ijsem/10.1099/ijsem.0.001544
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error