1887

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Volume 66, Issue 12

gen. nov., sp. nov., a psychrotolerant, -amino-acid-utilizing anaerobe isolated from two geographic locations of the Southern Hemisphere Free

Abstract

A Gram-stain-positive bacterium, strain LZ-22, was isolated from a rhizosphere of moss sp. collected at the shore of Lake Zub in Antarctica. Cells were motile, straight or pleomorphic rods with sizes of 0.6–1.0×3.5–10 µm. The novel isolate was a facultatively anaerobic, catalase-positive, psychrotolerant mesophile. Growth was observed at 3–41 °C (optimum 24–28 °C), with 0–7 % (w/v) NaCl (optimum 0.25 %) and at pH 4.0–9.0 (optimum pH 7.8). The quinone system of strain LZ-22 possessed predominately menaquinone MK-9(H). The genomic G+C content was 70.2 mol%. Strain 10J was isolated from a biofilm of sediment microbial fuel cell, in Uruguay and had 99 % 16S rRNA gene sequence similarity to strain LZ-22. DNA–DNA-hybridization values of 84 % confirmed that both strains belonged to the same species. Both strains grew on sugars, proteinaceous compounds, and some amino- and organic acids. Strain LZ-22 uniquely grew on D-enantiomers of histidine and valine while neglecting growth on L-enantiomers. Both strains were sensitive to most of the tested antibiotics but resistant to tested nitrofurans and sulfanilamides. Phylogenetic analyses of the 16S rRNA gene sequences indicated that the strains were related to members of the family (~93–94 % 16S rRNA gene sequence similarity) with formation of a separate branch within the radiation of the genera and . Based on phenotypic and genotypic characteristics, we propose the affiliation of both strains into a novel species of a new genus. The name gen. nov., sp. nov. is proposed for the novel taxon with the type strain LZ-22 (=ATCC TSD-18=DSM 100494=JCM 30886).

  • Received:
  • Accepted:
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Keyword(s): anaerobe , D-amino acids , polymorphic rods , polyphosphate granules , Propionibacteriaceae and psychrotolerant
© 2016 IUMS
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Raineyella antarctica gen. nov., sp. nov., a psychrotolerant, d-amino-acid-utilizing anaerobe isolated from two geographic locations of the Southern Hemisphere
Int J Syst Evol Microbiol 66, 5529 (2016); https://doi.org/10.1099/ijsem.0.001552
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