RT Journal Article SR Electronic(1) A1 Park, Sooyeon A1 Yoon, Sun Young A1 Ha, Min-Ji A1 Jung, Yong-Taek A1 Yoon, Jung-HoonYR 2016 T1 Primorskyibacteraestuariivivens sp. nov., isolated from a tidal flat, and emended description of the genus Primorskyibacter JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 12 SP 5550 OP 5555 DO https://doi.org/10.1099/ijsem.0.001555 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, aerobic, non-spore-forming, motile and coccoid, ovoid or rod-shaped bacterial strain, designated OITF-36T, was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-36T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain OITF-36T clustered with the type strains of Primorskyibacter sedentarius and Primorskyibacter insulae , showing 97.4–97.6 % sequence similarity. It exhibited sequence similarity values of less than 96.9 % to the type strains of the other recognized species. Strain OITF-36T contained Q-10 as the predominant ubiquinone and C18 : 1 ω7c as the major fatty acid. The major polar lipids detected in strain OITF-36T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain OITF-36T was 61.8 mol%, and its mean DNA–DNA relatedness values with the type strains of P . sedentarius and P . insulae were 16–24 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain OITF-36T is separate from P . sedentarius and P . insulae . On the basis of the data presented, strain OITF-36T is considered to represent a novel species of the genus Primorskyibacter , for which the name Primorskyibacter aestuariivivens sp. nov. is proposed. The type strain is OITF-36T (=KCTC 52455T=NBRC 112419T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001555