1887

Abstract

Gram-stain-negative strains V5/3M, V5/5K, V5/5M and V5/13 were isolated from root nodules of plants growing in the North Ossetia region (Caucasus). Sequencing of the partial 16S rRNA gene () and four housekeeping genes (, , and ) showed that the isolates from were most closely related to the species (order family ) which was described for the single isolate from root nodule of growing in Zambia. Sequence similarities between the -related isolates and WSM3693 ranged from 98.5 to 98.7 % for and from 79.7 to 95.8 % for housekeeping genes. Cellular fatty acids of the isolates V5/3M, V5/5K, V5/5M and V5/13 included important amounts of Cω7 (54.0–67.2 %), C (6.0–7.8 %), C cyclo ω8 (3.1–10.2 %), summed feature 2 (comprising one or more of iso-C I, C 3-OH and unknown ECL 10.938, 5.8–22.5 %) and summed feature 3 (comprising Cω7 and/or iso-C2-OH, 2.9–4.0 %). DNA–DNA hybridization between the isolate V5/3M and WSM3693 revealed DNA–DNA relatedness of 35.3 %. Analysis of morphological and physiological features of the novel isolates demonstrated their unique phenotypic profile in comparison with reference strains from closely related species of the genus . On the basis of genotypic and phenotypic analysis, a novel species named sp. nov. is proposed. The type strain is V5/3M (=LMG 29787=RCAM 02728). Three additional strains of the species are V5/5K, V5/5M and V5/13.

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2017-01-01
2024-03-29
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