1887

Abstract

A Gram-stain-negative, non-motile, non-spore-forming, aerobic and short rod-shaped bacterial strain, CAU 1311, was isolated from sea sand in the Republic of Korea. Strain CAU 1311 grew at temperatures from 20–37 °C, in the range of pH 6.5–10.0, and under various NaCl concentrations from 0–6 % (w/v). Phylogenetic analysis based on the 16S rRNA gene sequence of CAU 1311 showed that it formed a distinct lineage within the family as a separate deep branch, with 96.2 % or lower sequence identity to representatives of the genera , , , and . The major fatty acid was C 7 and the predominant respiratory quinone was Q-10. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid and an unidentified glycolipid. The DNA G+C content was 60.7 mol%. On the basis of genotypic, phenotypic and chemotaxanomic findings, strain CAU 1311 could be classified as representing a novel species of a new genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is CAU 1311 (=KCTC 52189=NBRC 111988).

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2017-01-01
2024-04-20
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References

  1. Williams KP, Sobral BW, Dickerman AW. A robust species tree for the Alphaproteobacteria. J Bacteriol 2007; 189:4574–4589
    [Google Scholar]
  2. Buchan A, González JM, Moran MA. Overview of the marine Roseobacter lineage. Appl Environ Microbiol 2005; 71:5665–5677 [View Article][PubMed]
    [Google Scholar]
  3. Garrity GM, Bell JA, Lilburn T. Order III. Rhodobacterales ord. nov. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed. vol. 2, part C New York: Springer; 2005 p. 161
    [Google Scholar]
  4. Garrity GM, Bell JA, Lilburn T. List of new names and new combinations previously effectively, but not validly, published. Int J Syst Evol Microbiol 2006; 56:1–6 [View Article][PubMed]
    [Google Scholar]
  5. Zhang G, Yang Y, Wang S, Sun Z, Jiao K. Alkalimicrobium pacificum gen. nov., sp. nov., a marine bacterium in the family Rhodobacteraceae. Int J Syst Evol Microbiol 2015; 65:2453–2458 [View Article][PubMed]
    [Google Scholar]
  6. Li AH, Zhou YG. Frigidibacter albus gen. nov., sp. nov., a novel member of the family Rhodobacteraceae isolated from lake water. Int J Syst Evol Microbiol 2015; 65:1199–1206 [View Article][PubMed]
    [Google Scholar]
  7. Sun F, Du Y, Liu X, Lai Q, Shao Z. Halovulum dunhuangense gen. nov., sp. nov., isolated from a saline terrestrial spring. Int J Syst Evol Microbiol 2015; 65:2810–2816 [View Article][PubMed]
    [Google Scholar]
  8. Zhong ZP, Liu Y, Wang F, Zhou YG, Liu HC et al. Lacimonas salitolerans gen. nov., sp. nov., isolated from surface water of a saline lake. Int J Syst Evol Microbiol 2015; 65:4550–4556 [View Article][PubMed]
    [Google Scholar]
  9. Park S, Won SM, Park JM, Jung YT, Yoon JH. Pontivivens insulae gen. nov., sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2015; 65:2896–2902 [View Article][PubMed]
    [Google Scholar]
  10. Won SM, Park S, Park JM, Kim BC, Yoon JH. Pseudohalocynthiibacter aestuariivivens gen. nov., sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 2015; 65:1509–1514 [View Article][PubMed]
    [Google Scholar]
  11. Park S, Park JM, Kang CH, Kim SG, Yoon JH. Pseudoseohaeicola caenipelagi gen. nov., sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 2015; 65:1819–1824 [View Article][PubMed]
    [Google Scholar]
  12. Billerbeck S, Orchard J, Tindall BJ, Giebel HA, Brinkhoff T et al. Description of Octadecabacter temperatus sp. nov., isolated from the southern North Sea, emended descriptions of the genus Octadecabacter and its species and reclassification of Octadecabacter jejudonensis Park and Yoon 2014 as Pseudooctadecabacter jejudonensis gen. nov., comb. nov. Int J Syst Evol Microbiol 2015; 65:1967–1974 [View Article][PubMed]
    [Google Scholar]
  13. Park S, Ha MJ, Jung YT, Yoon JH. Litorisediminivivens gilvus gen. nov., sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 2016; 66:4681–4685 [View Article][PubMed]
    [Google Scholar]
  14. Jung YT, Lee JS, Yoon JH. Maliponia aquimaris gen. nov., sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2016; 66:2271–2277 [View Article][PubMed]
    [Google Scholar]
  15. Park S, Jung YT, Yoon JH. Pseudoroseicyclus aestuarii gen. nov., sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 2016; 66:2165–2171 [View Article][PubMed]
    [Google Scholar]
  16. Crenn K, Serpin D, Lepleux C, Overmann J, Jeanthon C. Silicimonas algicola gen. nov., sp. nov., a member of the Roseobacter clade isolated from the cell surface of the marine diatom Thalassiosira delicatula. Int J Syst Evol Microbiol 2016; 66:4580–4588 [View Article][PubMed]
    [Google Scholar]
  17. Wang L, Liu Y, Shi X, Wang Y, Zheng Y et al. Xuhuaishuia manganoxidans gen. nov., sp. nov., a manganese-oxidizing bacterium isolated from deep-sea sediments from the Pacific Polymetallic Nodule Province. Int J Syst Evol Microbiol 2016; 66:1521–1526 [View Article][PubMed]
    [Google Scholar]
  18. Gordon RE, Mihm JM. Identification of Nocardia caviae (Erikson) nov. comb. Ann N Y Acad Sci 1962; 98:628–636 [View Article]
    [Google Scholar]
  19. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961; 3:208–218 [View Article]
    [Google Scholar]
  20. Lane DJ. 16S/23S RNA sequencing. In: Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics London: John Wiley & Sons Ltd; 1991 pp. 115–175
    [Google Scholar]
  21. Larkin MA, Blackshields G, Brown NP, Chenna R, Mcgettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007; 23:2947–2948 [View Article][PubMed]
    [Google Scholar]
  22. Jukes TH, Cantor CR. Evolution of protein molecules. In: Munro HH. editor Mammalian Protein Metabolism New York: Academic Press; 1969 pp. 21–132 [CrossRef]
    [Google Scholar]
  23. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  24. Fitch WM, Margoliash E. Construction of phylogenetic trees. Science 1967; 155:279–284 [View Article][PubMed]
    [Google Scholar]
  25. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  26. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
    [Google Scholar]
  27. Felsenstein J. PHYLIP – phylogeny inference package (version 3.2). Cladistics 1989; 5:164–166
    [Google Scholar]
  28. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
    [Google Scholar]
  29. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984; 25:125–128 [View Article]
    [Google Scholar]
  30. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50:1861–1868 [View Article][PubMed]
    [Google Scholar]
  31. Park S, Won SM, Kim H, Park DS, Yoon JH. Aestuariivita boseongensis gen. nov., sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 2014; 64:2969–2974 [View Article][PubMed]
    [Google Scholar]
  32. Cappuccino JG, Sherman N. Microbiology: A Laboratory Manual, 6th ed. Menlo Park, CA: Benjamin/Cummings; 2002
    [Google Scholar]
  33. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987; 19:1–67 [CrossRef]
    [Google Scholar]
  34. Smibert RM, Krieg NR. Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
    [Google Scholar]
  35. Biemer JJ. Antimicrobial susceptibility testing by the Kirby-Bauer disc diffusion method. Ann Clin Lab Sci 1973; 3:135–140[PubMed]
    [Google Scholar]
  36. Minnikin DE, Hutchinson IG, Caldicott AB, Goodfellow M. Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 1980; 188:221–233 [View Article]
    [Google Scholar]
  37. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–208 [CrossRef]
    [Google Scholar]
  38. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  39. Labrenz M, Tindall BJ, Lawson PA, Collins MD, Schumann P et al. Staleya guttiformis gen. nov., sp. nov. and Sulfitobacter brevis sp. nov., α-3-Proteobacteria from hypersaline, heliothermal and meromictic Antarctic Ekho Lake. Int J Syst Evol Microbiol 2000; 50:303–313 [View Article][PubMed]
    [Google Scholar]
  40. Xu H, Jiang L, Li S, Zeng X, Shao Z. Mameliella atlantica sp. nov., a marine bacterium of the Roseobacter clade isolated from deep-sea sediment of the South Atlantic Ocean. Int J Syst Evol Microbiol 2015; 65:2255–2259 [View Article][PubMed]
    [Google Scholar]
  41. Hong Z, Lai Q, Luo Q, Jiang S, Zhu R et al. Sulfitobacter pseudonitzschiae sp. nov., isolated from the toxic marine diatom Pseudo-nitzschia multiseries. Int J Syst Evol Microbiol 2015; 65:95–100 [View Article][PubMed]
    [Google Scholar]
  42. Zhong ZP, Liu Y, Zhou YG, Liu HC, Wang F et al. Maritimibacter lacisalsi sp. nov., isolated from a salt lake, and emended description of the genus Maritimibacter Lee et al. 2007. Int J Syst Evol Microbiol 2015; 65:3462–3468 [View Article][PubMed]
    [Google Scholar]
  43. Lee K, Choo YJ, Giovannoni SJ, Cho JC. Maritimibacter alkaliphilus gen. nov., sp. nov., a genome-sequenced marine bacterium of the Roseobacter clade in the order Rhodobacterales. Int J Syst Evol Microbiol 2007; 57:1653–1658 [View Article][PubMed]
    [Google Scholar]
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