1887

Abstract

A novel endophytic actinobacterial strain, designated EGI 6500195, was isolated from fruits of . Growth occurred at 10–45 °C (optimum 30 °C), at pH 6–8 (optimum pH 7) and in the presence of 0–1 % (w/v) NaCl. Strain EGI 6500195 shared highest 16S rRNA gene sequence similarity (97.74 %) with DSM 41686 and less than 97 % sequence similarity with other members of the genus . The diagnostic amino acid in the peptidoglycan was -diaminopimelic acid. Whole-cell hydrolysates contained glucose, ribose, fructose and mannose. The predominant menaquinones were MK-9(H) and MK-9(H). The polar lipid profile of strain EGI 6500195 included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylcholine, three unknown phospholipids, an unknown aminophospholipid and an unknown aminolipid. The cellular fatty acids were anteiso-C, anteiso-C, iso-C, iso-C, anteiso-C 9, summed feature 4 (iso-C I and/or anteiso-C B) and iso-C 9. The DNA G+C content of strain EGI 6500195 was 74.1 mol%. The level of DNA–DNA relatedness between strain EGI 6500195 and DSM 41686 was 14.1±3.5 %. On the basis of the phenotypic, phylogenetic, chemotaxonomic and DNA–DNA hybridization data, strain EGI 6500195 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EGI 6500195 (=DSM 42145=JCM 30089).

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2017-01-01
2024-03-28
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