1887

Abstract

Three yeast isolates, G5-5(5), G5-9(3) and G5-9(4), were obtained from the sugar cane juice and waste from sugar production plant (Korach Industry Co., Ltd) in Korach province, Thailand. They were found to belong to the same species based on DNA sequence identity of the small subunit ribosomal RNA gene (SSU) and the D1/D2 region of the large subunit rRNA gene (LSU D1/D2). A n search of the GenBank database revealed they had 93 % nucleotide sequence identity to for the SSU (1742 bp), but their LSU D1/D2 sequence (572 bp) showed less than 90 % identity to all available sequences in the database. Phylogenetic analyses with neighbour-joining and maximum-parsimony methods using the aligned LSU D1/D2 and SSU sequences (a total of 2072 positions after removal of gaps) inferred that the three isolates were separated from all known taxa in the Saccharomycetales, and that the neighbouring taxa were species of /. Physiological and biochemical characters revealed distinct differences between the three isolates and / species, including the ability to assimilate several carbon sources and inability to ferment glucose. Thus, isolates G5-5(5), G5-9(3) and G5-9(4) should be assigned to a novel taxon, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is G5-5(5) (=CBS 14167=JCM 18455=TISTR 5950), with MycoBank number MB815477 (for the genus) and MB817751 (for the species). Two additional strains of the species are G5-9(3) (=JCM 18456) and G5-9(4) (=JCM 18457).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001607
2017-02-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/2/250.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001607&mimeType=html&fmt=ahah

References

  1. Imanishi Y, Jindamorakot S, Mikata K, Nakagiri A, Limtong S et al. Two new ascomycetous anamorphic yeast species related to Candida friedrichiiCandida jaroonii sp. nov., and Candida songkhlaensis sp. nov.—isolated in Thailand. Antonie van Leeuwenhoek 2008; 94:267–276 [View Article][PubMed]
    [Google Scholar]
  2. Koowadjanakul N, Jindamorakot S, Yongmanitchai W, Limtong S. Ogataea phyllophila sp. nov., Candida chumphonensis sp. nov. and Candida mattranensis sp. nov., three methylotrophic yeast species from phylloplane in Thailand. Antonie van Leeuwenhoek 2011; 100:207–217 [View Article][PubMed]
    [Google Scholar]
  3. Nakase T, Jindamorakot S, Am-In S, Ninomiya S, Kawasaki H et al. Candida maleeae sp. nov., a novel anamorphic yeast species in the Ambrosiozyma clade found in Thailand. J Gen Appl Microbiol 2011; 57:253–258[PubMed] [CrossRef]
    [Google Scholar]
  4. Nakase T, Jindamorakot S, Am-In S, Ninomiya S, Kawasaki H. Wickerhamomyces tratensis sp. nov. and Candida namnaoensis sp. nov., two novel ascomycetous yeast species in the Wickerhamomyces clade found in Thailand. J Gen Appl Microbiol 2012; 58:145–152[PubMed] [CrossRef]
    [Google Scholar]
  5. Kaewwichian R, Yongmanitchai W, Kawasaki H, Limtong S. Metschnikowia saccharicola sp. nov. and Metschnikowia lopburiensis sp. nov., two novel yeast species isolated from phylloplane in Thailand. Antonie van Leeuwenhoek 2012; 102:743–751 [View Article][PubMed]
    [Google Scholar]
  6. Kaewwichian R, Kawasaki H, Limtong S. Wickerhamomyces siamensis sp. nov., a novel yeast species isolated from the phylloplane in Thailand. Int J Syst Evol Microbiol 2013; 63:1568–1573 [View Article][PubMed]
    [Google Scholar]
  7. Saksinchai S, Suzuki M, Chantawannakul P, Ohkuma M, Lumyong S. A novel ascosporogenous yeast species, Zygosaccharomyces siamensis, and the sugar tolerant yeasts associated with raw honey collected in Thailand. Fungal Diversity 2012; 52:123–139 [CrossRef]
    [Google Scholar]
  8. Chamnanpa T, Limtong P, Srisuk N, Limtong S. Pseudozyma vetiver sp. nov., a novel anamorphic ustilaginomycetous yeast species isolated from the phylloplane in Thailand. Antonie van Leeuwenhoek 2013; 104:637–644 [View Article][PubMed]
    [Google Scholar]
  9. Lorliam W, Akaracharanya A, Suzuki M, Ohkuma M, Tanasupawat S. Diversity and fermentation products of xylose-utilizing yeasts isolated from buffalo feces in Thailand. Microbes Environ 2013; 28:354–360 [View Article][PubMed]
    [Google Scholar]
  10. Khunnamwong P, Surussawadee J, Jindamorakot S, Limtong S. Wickerhamiella siamensis f.a., sp. nov., an endophytic and epiphytic yeast species isolated from sugar cane leaf. Int J Syst Evol Microbiol 2014; 64:3849–3855 [View Article][PubMed]
    [Google Scholar]
  11. Khunnamwong P, Surussawadee J, Jindamorakot S, Ribeiro JR, Hagler AN et al. Occultifur tropicalis f.a., sp. nov., a novel cystobasidiomycetous yeast species isolated from tropical regions. Int J Syst Evol Microbiol 2015; 65:1578–1582 [View Article][PubMed]
    [Google Scholar]
  12. FAOSTAT 2012; Food and Agricultural Organization of United Nations (FAO). http://faostat.fao.org/site/339/default.aspx
  13. Jutakanoke R, Tanasupawat S, Akaracharanya A. Characterization and ethanol fermentation of Pichia and Torulaspora strains. J App Pharm Sci 2014; 4:52–56
    [Google Scholar]
  14. Laopaiboon L, Nuanpeng S, Srinophakun P, Klanrit P, Laopaiboon P. Ethanol production from sweet sorghum juice using very high gravity technology: Effect of carbon and nitrogen supplementations. Bioresource Technol 2009; 100:4176–4182 [CrossRef]
    [Google Scholar]
  15. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurzman CP, Fell JW, Boekhout T. (editors) The Yeasts: A Taxonomic Study, 5th ed. vol. 1 San Diego, CA: Elsevier; 201197–106
    [Google Scholar]
  16. Nakase T, Suzuki M. Taxonomic studies on Debaryomyces hansenii (Zopf) Lodder et Kreger-Van Rij and related species. I. Chemotaxonomic investigations. J Gen Appl Microbiol 1985; 31:49–69 [CrossRef]
    [Google Scholar]
  17. Maniatis T, Fritsch EF, Sambrook J. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 1982
    [Google Scholar]
  18. Fell JW, Boekhout T, Fonseca A, Scorzetti G, Statzell-Tallman A. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 2000; 50:1351–1371 [View Article][PubMed]
    [Google Scholar]
  19. Noda H, Nakashima N, Koizumi M. Phylogenetic position of Yeast-like symbiotes of rice planthoppers based on partial 18S rDNA sequences. Insect Biochem Mol Biol 1995; 25:639–646 [CrossRef]
    [Google Scholar]
  20. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: A Guide to Methods and Applications New York: Academic Press, Inc; 1990315–322
    [Google Scholar]
  21. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  22. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  23. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120[PubMed] [CrossRef]
    [Google Scholar]
  24. Nei M, Kumar S. Molecular Evolution and Phylogenetics New York: Oxford University Press; 2000 p 126
    [Google Scholar]
  25. Kurtzman CP, Robnett CJ. Relationships among genera of the Saccharomycotina (Ascomycota) from multigene phylogenetic analysis of type species. FEMS Yeast Res 2013; 13:23–33 [View Article][PubMed]
    [Google Scholar]
  26. Takashima M, Sugita T, Van BH, Nakamura M, Endoh R et al. Taxonomic richness of yeasts in Japan within subtropical and cool temperate areas. PLoS One 2012; 7:e50784 [View Article][PubMed]
    [Google Scholar]
  27. Smith MT. Dekkera van der Walt (1964). In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts: A Taxonomic Study, 5th ed. vol. 2 Amsterdam: Elsevier; 2011373–377 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001607
Loading
/content/journal/ijsem/10.1099/ijsem.0.001607
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error