@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001616, author = "Wang, Hong-Fei and Li, Qiu-Li and Zhang, Yong-Guang and Xiao, Min and Zhou, Xing-Kui and Guo, Jian-Wei and Duan, Yan-Qing and Li, Wen-Jun", title = "Bacillus capparidis sp. nov., an endophytic bacterium isolated from roots of Capparis spinosa L.", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "2", pages = "282-287", doi = "https://doi.org/10.1099/ijsem.0.001616", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001616", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "endophytic bacterium", keywords = "Capparis spinosa L", keywords = "Bacillus capparidis sp. nov.", abstract = "A novel endophytic bacterium, designated strain EGI 6500252T, was isolated from the surface-sterilized roots of a medicinal plant (Capparis spinosa L.) collected from Urumqi city, Xinjiang, north-west China. Cells were Gram-stain-positive, non-motile, aerobic, catalase- and oxidase-positive, rod-shaped and did not display spore formation. Strain EGI 6500252T grew at 10–40 °C (optimum 25–30 °C), at pH 6.0–8.0 (optimum pH 7.0) and in the presence of 0–10 % (w/v) NaCl (optimum 0–3 %). The major cellular fatty acids (>10 %) were identified as iso-C15 : 0, anteiso-C15 : 0, anteiso-C17 : 0 and summed feature 4. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, four unknown phospholipids, one unknown glycolipid and one unknown lipid. The dominant isoprenoid quinone was menaquinone 7 (MK-7). The DNA G+C content was 39.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain EGI 6500252T belonged to the genus Bacillus , and exhibited a highest 16S rRNA gene sequence similarity (96.2 %) that was lower than the suggested threshold (97.0 %) for separating bacterial species. On the basis of the phylogenetic analysis, chemotaxonomic data and physiological characteristics, strain EGI 6500252T represents a novel species of the genus Bacillus , for which the name Bacillus capparidis sp. nov. is proposed. The type strain is EGI 6500252T (=CGMCC 1.12820T=KCTC 33514T).", }