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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 3

Rapid and accurate identification of species of the genus isolated from Korean fermented foods by matrix-assisted laser desorption/ionization time-of-flight MS with local database extension Free

Abstract

Pediococci are halophilic lactic acid bacteria, within the family , which are involved in the fermentation of various salted and fermented foods, such as kimchi and jeotgal. In this study, a matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS method was developed for the rapid identification of species of the genus . Of the 130 spectra aligned with the Biotyper taxonomy database, 122 isolates (93.9 %) yielded log scores <1.7, which means they were not identifiable. After registering the spectra of 11 reference strains of the genus , all of the isolates were correctly identified, of which 84 (64.6 %) and 46 (35.4 %) were identified at the species and genus level, respectively. In comparing food origins, no relationship was found between the bacterial characteristics and food environment. We were able to produce a Biotyper system for identification of members of the genus with locally extended reference strains. The MALDI-TOF MS method is fast, simple and reliable for discriminating between species in the genus and therefore will be useful for quality control in determining the spoilage of alcoholic beverages or in the production of fermented food.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): fermented food , jeotgal , kimchi , MALDI-TOF MS and Pediococcus
© 2017 IUMS
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2017-03-01
2024-04-18
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Rapid and accurate identification of species of the genus Pediococcus isolated from Korean fermented foods by matrix-assisted laser desorption/ionization time-of-flight MS with local database extension
Int J Syst Evol Microbiol 67, 744 (2017); https://doi.org/10.1099/ijsem.0.001626
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