%0 Journal Article %A Hahn, Martin W. %A Huymann, Lesley R. %A Koll, Ulrike %A Schmidt, Johanna %A Lang, Elke %A Hoetzinger, Matthias %T Polynucleobacter wuianus sp. nov., a free-living freshwater bacterium affiliated with the cryptic species complex PnecC %D 2017 %J International Journal of Systematic and Evolutionary Microbiology, %V 67 %N 2 %P 379-385 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001637 %K Polynucleobacter %K average nucleotide identity %K genome %K multi locus sequence tree %K Burkholderiaceae %I Microbiology Society, %X Strain QLW-P1FAT50C-4T, isolated from a shallow, acidic freshwater pond located in the Austrian Alps at an altitude of 1300 m, was characterized by investigation of phenotypic, chemotaxonomic and genomic traits. As shown previously, phylogenetic analyses based on 16S rRNA gene sequences placed the strain in the cryptic species complex PnecC within the genus Polynucleobacter . The major fatty acids of the strain were C16 : 1ω7c and C18 : 1ω7c. The strain has a genome of 2.23 Mbp with a DNA G+C content of 44.9 mol%. The strain encodes a seemingly complete gene cluster for anoxygenic photosynthesis but lacks typical genes for CO2 assimilation. In order to resolve the phylogenetic position of the strain within the species complex PnecC, concatenated partial sequences of eight housekeeping genes were analysed. The phylogenetic reconstruction obtained did not place strain QLW-P1FAT50C-4T close to any of the five previously described species within subcluster PnecC. Pairwise average nucleotide identity (ANI) comparisons of whole-genome sequences suggested that strain QLW-P1FAT50C-4T (=DSM 24008T=CIP 111100T) represents a novel species, for which we propose the name Polynucleobacter wuianus sp. nov. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001637